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PMID: 7892244 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Enhanced activity of the bacteriophage lambda PL promoter at low temperature.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 6 ·1995-03-14 ·Pages 2184-8

Giladi H, Goldenberg D, Koby S, Oppenheim AB

Abstract

The response of the early phage lambda PL promoter to temperature was investigated. Experiments with lacZ reporter gene fusions demonstrated that the activity of the phage lambda PL promoter is inversely dependent on temperature. The bacterial DNA-binding protein integration host factor (IHF) further enhances lambda PL promoter activity at low temperature, although no apparent changes in the cellular level of IHF protein were observed at the different temperatures. IHF protein binds DNA in vitro more avidly at low temperatures. In vitro transcription assays further revealed that the temperature response of PL is the result of an intrinsic property of the promoter as well as its activation by IHF.

MeSH Terms
Bacterial Proteins/biosynthesis,metabolism Bacteriophage lambda/genetics,metabolism Base Sequence Cold Temperature DNA Primers DNA-Binding Proteins/metabolism Escherichia coli/genetics,metabolism Gene Expression Regulation, Viral Integration Host Factors Kinetics Molecular Sequence Data Polymerase Chain Reaction Promoter Regions, Genetic Recombinant Fusion Proteins/biosynthesis Recombination, Genetic Thermodynamics Transcription, Genetic beta-Galactosidase/biosynthesis
Chemicals
Bacterial Proteins DNA Primers DNA-Binding Proteins Integration Host Factors Recombinant Fusion Proteins beta-Galactosidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Giladi H
Department of Molecular Genetics, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Goldenberg D
Koby S
Oppenheim A B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.6.2184
Published
1995-03-14
Pages
2184-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC42448
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.92.6.2184 PMC Full Text View journal details
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