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PMID: 7882973 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A novel type of myosin implicated in signalling by rho family GTPases.

The EMBO journal ·Vol. 14 ·No. 4 ·1995-02-15 ·Pages 697-704

Reinhard J, Scheel AA, Diekmann D, Hall A, Ruppert C, Bähler M

Abstract

A novel widely expressed type of myosin (fifth unconventional myosin from rat: myr 5) from rat tissues, defining a ninth class of myosins, was identified. The predicted amino acid sequence of myr 5 exhibits several features not found previously in myosins. The myosin head domain contains a unique N-terminal extension and an insertion of 120 amino acids at a postulated myosin-actin contact site. Nevertheless, myr 5 is able to bind actin filaments in an ATP-regulated manner. The head domain is followed by four putative light chain binding sites. The tail domain of myr 5 contains a region which coordinates two atoms of zinc followed by a region that stimulates GTP hydrolysis of members of the ras-related rho subfamily of small G-proteins. Myr 5 therefore provides the first direct link between rho GTPases which have been implicated in the regulation of actin organization and the actin cytoskeleton. It is also the first unconventional myosin for which a tail binding partner(s), namely members of the rho family, has been identified.

Related Genes
Abr Bcr DGK-1 DGK-2 PKC RhoGAP chim myr5 raf-1 rotund unc13 vav
MeSH Terms
Actins/metabolism Amino Acid Sequence Animals GTP Phosphohydrolases/genetics GTP-Binding Proteins/metabolism Molecular Sequence Data Myosins/chemistry,classification,genetics,metabolism Phorbol Esters/metabolism Protein Binding Rats Sequence Alignment Sequence Homology, Amino Acid Signal Transduction Zinc/metabolism
Chemicals
Actins Myo1e protein, rat Phorbol Esters GTP Phosphohydrolases GTP-Binding Proteins Myosins Zinc
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Reinhard J
Friedrich-Miescher Laboratorium der Max-Planck-Gesellschaft, Tübingen, Germany.
Scheel A A
Diekmann D
Hall A
Ruppert C
Bähler M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-02-15
Pages
697-704
Language
English
Region
England
NLM ID
8208664
PMCID
PMC398134
Subset
IM
Databases
GENBANK
X77609
External Links
PubMed source PMC Full Text View journal details
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