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PMID: 7878051 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Translocation of the Escherichia coli transcription complex observed in the registers 11 to 20: "jumping" of RNA polymerase and asymmetric expansion and contraction of the "transcription bubble".

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 5 ·1995-02-28 ·Pages 1739-43

Zaychikov E, Denissova L, Heumann H

Abstract

Translocation of DNA-dependent RNA polymerase along the DNA template during RNA synthesis encompasses continuous as well as discontinuous steps. This is demonstrated by chemical probing of transcription complexes stalled in consecutive registers of RNA synthesis at base positions +11, +12, +14, +16, +18, and +20. The "transcription bubble" translocates by continuous opening of the downstream edge in tandem with the growing RNA chain and discontinuous closing at the upstream edge after at least nine steps of RNA synthesis. The position of the enzyme remains unchanged during extension of the transcription bubble and "jumps" 10 bp downstream simultaneously with collapse of the transcription bubble.

MeSH Terms
Base Sequence DNA-Binding Proteins/metabolism DNA-Directed RNA Polymerases/metabolism Escherichia coli/genetics Kinetics Molecular Sequence Data Promoter Regions, Genetic Templates, Genetic Transcription, Genetic
Chemicals
DNA-Binding Proteins DNA-Directed RNA Polymerases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zaychikov E
Max Planck Institute of Biochemistry, Martinsried, Germany.
Denissova L
Heumann H
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28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.5.1739
Published
1995-02-28
Pages
1739-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC42595
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.92.5.1739 PMC Full Text View journal details
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