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PMID: 3072199 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA-dependent RNA polymerase of Escherichia coli induces bending or an increased flexibility of DNA by specific complex formation.

The EMBO journal ·Vol. 7 ·No. 13 ·1988-12-20 ·Pages 4379-81

Heumann H, Ricchetti M, Werel W

Abstract

Escherichia coli RNA polymerase is shown to induce bending or an increased flexibility of the promoter DNA. This is a specific effect of holoenzyme (core enzyme and sigma-factor). The centre of the flexibility is 3 bp upstream of the initiation point of RNA synthesis. This flexibility or bending is maintained during RNA synthesis by core enzyme.

MeSH Terms
DNA, Bacterial/genetics,metabolism DNA-Directed RNA Polymerases/metabolism Escherichia coli/genetics,metabolism Genes, Bacterial Nucleic Acid Conformation Promoter Regions, Genetic Transcription, Genetic
Chemicals
DNA, Bacterial DNA-Directed RNA Polymerases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Heumann H
Max-Planck-Institut für Biochemie, Martinsried, FRG.
Ricchetti M
Werel W
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26 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-12-20
Pages
4379-81
Language
English
Region
England
NLM ID
8208664
PMCID
PMC455170
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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