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PMID: 7868621 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The nasB operon and nasA gene are required for nitrate/nitrite assimilation in Bacillus subtilis.

Journal of bacteriology ·Vol. 177 ·No. 5 ·1995-03-00 ·Pages 1409-13

Ogawa K, Akagawa E, Yamane K, Sun ZW, LaCelle M, Zuber P, Nakano MM

Abstract

Bacillus subtilis can use either nitrate or nitrite as a sole source of nitrogen. The isolation of the nasABCDEF genes of B. subtilis, which are required for nitrate/nitrite assimilation, is reported. The probable gene products include subunits of nitrate/nitrite reductases and an enzyme involved in the synthesis of siroheme, a cofactor for nitrite reductase.

MeSH Terms
Anion Transport Proteins Bacillus subtilis/genetics,metabolism Bacterial Proteins/genetics Carrier Proteins/genetics Chromosome Walking Genes, Bacterial/genetics Nitrate Reductase Nitrate Reductases/genetics Nitrate Transporters Nitrates/metabolism Nitrites/metabolism Open Reading Frames/genetics Operon/genetics Sequence Analysis, DNA Sequence Homology
Chemicals
Anion Transport Proteins Bacterial Proteins Carrier Proteins Nitrate Transporters Nitrates Nitrites Nitrate Reductases Nitrate Reductase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ogawa K
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
Akagawa E
Yamane K
Sun Z W
LaCelle M
Zuber P
Nakano M M
References (27)
27 references, click to expand
  1. Rapid and sensitive protein similarity searches.
    Science. 1985 Mar 22;227(4693):1435-41 PMID: 2983426
  2. Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1984 Jan;81(2):439-43 PMID: 6420789
  3. Nucleotide sequence and expression of the selenocysteine-containing polypeptide of formate dehydrogenase (formate-hydrogen-lyase-linked) from Escherichia coli.
    Proc Natl Acad Sci U S A. 1986 Jul;83(13):4650-4 PMID: 2941757
  4. Role of AbrB in Spo0A- and Spo0B-dependent utilization of a sporulation promoter in Bacillus subtilis.
    J Bacteriol. 1987 May;169(5):2223-30 PMID: 2437099
  5. Cloning of Streptococcus pneumoniae DNA fragments in Escherichia coli requires vectors protected by strong transcriptional terminators.
    Gene. 1987;55(2-3):179-87 PMID: 3311881
  6. Identification and expression of genes narL and narX of the nar (nitrate reductase) locus in Escherichia coli K-12.
    J Bacteriol. 1988 Apr;170(4):1589-97 PMID: 2832370
  7. Identification of a genetic locus required for biosynthesis of the lipopeptide antibiotic surfactin in Bacillus subtilis.
    J Bacteriol. 1988 Dec;170(12):5662-8 PMID: 2848009
  8. The narK gene product participates in nitrate transport induced in Escherichia coli nitrate-respiring cells.
    FEBS Lett. 1989 Jul 31;252(1-2):139-43 PMID: 2668029
  9. Nitrate reductase of Escherichia coli: completion of the nucleotide sequence of the nar operon and reassessment of the role of the alpha and beta subunits in iron binding and electron transfer.
    Mol Gen Genet. 1989 Aug;218(2):249-56 PMID: 2674654
  10. The Escherichia coli cysG gene encodes S-adenosylmethionine-dependent uroporphyrinogen III methylase.
    Biochem J. 1990 Feb 1;265(3):725-9 PMID: 2407234
  11. Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB-cysG region of the Escherichia coli K-12 chromosome.
    Eur J Biochem. 1990 Jul 31;191(2):315-23 PMID: 2200672
  12. Functional domains of assimilatory nitrate reductases and nitrite reductases.
    Trends Biochem Sci. 1990 Aug;15(8):315-9 PMID: 2204158
  13. Isolation and characterisation of the crnA-niiA-niaD gene cluster for nitrate assimilation in Aspergillus nidulans.
    Gene. 1990 Jun 15;90(2):181-92 PMID: 2205530
  14. Nucleotide sequence of a Pseudomonas denitrificans 5.4-kilobase DNA fragment containing five cob genes and identification of structural genes encoding S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase and cobyrinic acid a,c-diamide synthase.
    J Bacteriol. 1990 Oct;172(10):5968-79 PMID: 2211520
  15. Genetic evidence for interaction of sigma A with two promoters in Bacillus subtilis.
    J Bacteriol. 1991 Jun;173(11):3282-90 PMID: 1904429
  16. Primary structure, expression in Escherichia coli, and properties of S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase from Bacillus megaterium.
    J Bacteriol. 1991 Aug;173(15):4893-6 PMID: 1906874
  17. Transcriptional control, translation and function of the products of the five open reading frames of the Escherichia coli nir operon.
    Mol Microbiol. 1992 Oct;6(19):2805-13 PMID: 1435259
  18. Identification and characterization of a gene cluster involved in nitrate transport in the cyanobacterium Synechococcus sp. PCC7942.
    Mol Gen Genet. 1993 Jan;236(2-3):193-202 PMID: 8437564
  19. Structures of genes nasA and nasB, encoding assimilatory nitrate and nitrite reductases in Klebsiella pneumoniae M5al.
    J Bacteriol. 1993 Apr;175(8):2370-8 PMID: 8468296
  20. Molecular cloning, characterization, and nucleotide sequence of nit-6, the structural gene for nitrite reductase in Neurospora crassa.
    J Bacteriol. 1993 Apr;175(8):2379-92 PMID: 8096840
  21. The nasFEDCBA operon for nitrate and nitrite assimilation in Klebsiella pneumoniae M5al.
    J Bacteriol. 1994 May;176(9):2551-9 PMID: 8169203
  22. Nitrogen regulation of nasA and the nasB operon, which encode genes required for nitrate assimilation in Bacillus subtilis.
    J Bacteriol. 1995 Feb;177(3):573-9 PMID: 7836289
  23. Identification and isolation of a gene required for nitrate assimilation and anaerobic growth of Bacillus subtilis.
    J Bacteriol. 1995 Feb;177(4):1112-5 PMID: 7860592
  24. [Nitrate-reductase constitutivity for nitrate in early asporogenous mutants of Bacillus subtilus].
    Ann Inst Pasteur (Paris). 1970 Dec;119(6):711-8 PMID: 4993952
  25. Revision of the linkage map of Bacillus subtilis 168: indications for circularity of the chromosome.
    J Bacteriol. 1975 Mar;121(3):823-34 PMID: 803963
  26. Increased nitrate reductase A activity as a sign of membrane alteration in early blocked asporogenous mutants of Bacillus subtilis.
    Biochimie. 1976;58(1-2):99-108 PMID: 821543
  27. Molecular cloning and functional analysis of the cysG and nirB genes of Escherichia coli K12, two closely-linked genes required for NADH-dependent nitrite reductase activity.
    Mol Gen Genet. 1985;200(2):328-34 PMID: 2993824
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1995-03-00
Pages
1409-13
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC176753
Subset
IM
Grants
NIGMS NIH HHS · GM45898 · United States
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