Conjugation deficient E. coli K12 F- mutants with heptose-less lipopolysaccharide.

Molecular & general genetics : MGG ·Vol. 146 ·No. 1 ·1976-07-05 ·Pages 43-50

Havekes LM, Lugtenberg BJ, Hoekstra WP

Abstract

Two F- mutants deficient in conjugation with F-type donors are isolated and characterized. Phenotypically, these mutants are similar; they have heptose-less lipopolysaccharide and lack some outer membrane protein. Genotypically, they are different. One mutant harbors a point mutation in the 70 to 74 min region, while the other is deleted for the chromosomal region 6.5 to 8.5 min. Comparison of the properties of the conjugation-deficient mutants described in this paper with other such mutants suggests that an outer membrane protein is the receptor for the f-pilus.

MeSH Terms

Binding Sites Cell Membrane Chromosome Mapping Chromosomes, Bacterial Coliphages Conjugation, Genetic Escherichia coli/metabolism F Factor Genotype Heptoses/metabolism Lipopolysaccharides/metabolism Mutation Phenotype

Chemicals

Heptoses Lipopolysaccharides

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Havekes L M

Lugtenberg B J

Hoekstra W P

References (28)

28 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00267981

Published

1976-07-05

Pages

43-50

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00267981

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Conjugation deficient E. coli K12 F- mutants with heptose-less lipopolysaccharide.

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