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PMID: 4259664 Published · ppublish English Journal Article

Metabolism of pyrimidines and pyrimidine nucleosides by Salmonella typhimurium.

Journal of bacteriology ·Vol. 110 ·No. 1 ·1972-04-00 ·Pages 219-28

Beck CF, Ingraham JL, Neuhard J, Thomassen E

Abstract

The pathways by which uracil, cytosine, uridine, cytidine, deoxyuridine, and deoxycytidine are metabolized by Salmonella typhimurium are established. The various 5-fluoropyrimidine analogues are shown to exert their toxic effects only after having been converted to the nucleotide level, and these conversions are shown to be catalyzed by the same enzymes which similarly convert the natural substrates. Methods for isolating mutant strains blocked in various steps of metabolism of pyrimidine bases and nucleosides are described.

MeSH Terms
Aminohydrolases/analysis Cytidine/metabolism Cytosine/metabolism Deoxyuridine/metabolism Floxuridine/pharmacology Flucytosine/pharmacology Fluorouracil/pharmacology Genetics, Microbial Genotype Mutation Nucleosides/metabolism Nucleotidyltransferases/analysis Pentosyltransferases/analysis Phosphotransferases/analysis Pyrimidines/metabolism Salmonella typhimurium/drug effects,growth & development,metabolism Thymidine Uracil/metabolism Uracil Nucleotides Uridine/metabolism
Chemicals
Nucleosides Pyrimidines Uracil Nucleotides Floxuridine Uracil Cytidine Cytosine Flucytosine Pentosyltransferases Phosphotransferases Nucleotidyltransferases Aminohydrolases Fluorouracil Thymidine Deoxyuridine Uridine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Beck C F
Ingraham J L
Neuhard J
Thomassen E
References (23)
23 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.110.1.219-228.1972
Published
1972-04-00
Pages
219-28
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC247401
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.110.1.219-228.1972 PMC Full Text View journal details
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