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PMID: 7835332 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transport route for synaptobrevin via a novel pathway of insertion into the endoplasmic reticulum membrane.

The EMBO journal ·Vol. 14 ·No. 2 ·1995-01-16 ·Pages 217-23

Kutay U, Ahnert-Hilger G, Hartmann E, Wiedenmann B, Rapoport TA

Abstract

Synaptobrevin/vesicle-associated membrane protein is one of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins. It is proposed to provide specificity for the targeting and fusion of vesicles with the plasma membrane. It belongs to a class of membrane proteins which lack a signal sequence and contain a single hydrophobic segment close to their C-terminus, leaving most of the polypeptide chain in the cytoplasm (tail-anchored). We show that in neuroendocrine PC12 cells, synaptobrevin is not directly incorporated into the target organelle, synaptic-like vesicles. Rather, it is first inserted into the endoplasmic reticulum (ER) membrane and is then transported via the Golgi apparatus. Its insertion into the ER membrane in vitro occurs post-translationally, is dependent on ATP and results in a trans-membrane orientation of the hydrophobic tail. Membrane integration requires ER protein(s) different from the translocation components needed for proteins with signal sequences, thus suggesting a novel mechanism of insertion.

MeSH Terms
Adenosine Triphosphate/metabolism Amino Acid Sequence Animals Biological Transport Endopeptidases/metabolism Endoplasmic Reticulum/metabolism Intracellular Membranes/metabolism Membrane Proteins/metabolism Molecular Sequence Data Nerve Tissue Proteins/metabolism PC12 Cells Protein Processing, Post-Translational Proteolipids/metabolism R-SNARE Proteins Rats
Chemicals
Membrane Proteins Nerve Tissue Proteins Proteolipids R-SNARE Proteins proteoliposomes Adenosine Triphosphate Endopeptidases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kutay U
Max-Delbrück, Centre for Molecular Medicine, Berlin-Buch.
Ahnert-Hilger G
Hartmann E
Wiedenmann B
Rapoport T A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-01-16
Pages
217-23
Language
English
Region
England
NLM ID
8208664
PMCID
PMC398073
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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