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PMID: 1313437 Published · ppublish English Journal Article

The signal sequence receptor, unlike the signal recognition particle receptor, is not essential for protein translocation.

The Journal of cell biology ·Vol. 117 ·No. 1 ·1992-04-00 ·Pages 15-25

Migliaccio G, Nicchitta CV, Blobel G

Abstract

Detergent extracts of canine pancreas rough microsomal membranes were depleted of either the signal recognition particle receptor (SR), which mediates the signal recognition particle (SRP)-dependent targeting of the ribosome/nascent chain complex to the membrane, or the signal sequence receptor (SSR), which has been proposed to function as a membrane bound receptor for the newly targeted nascent chain and/or as a component of a multi-protein translocation complex responsible for transfer of the nascent chain across the membrane. Depletion of the two components was performed by chromatography of detergent extracts on immunoaffinity supports. Detergent extracts lacking either SR or SSR were reconstituted and assayed for activity with respect to SR dependent elongation arrest release, nascent chain targeting, ribosome binding, secretory precursor translocation, and membrane protein integration. Depletion of SR resulted in the loss of elongation arrest release activity, nascent chain targeting, secretory protein translocation, and membrane protein integration, although ribosome binding was unaffected. Full activity was restored by addition of immunoaffinity purified SR before reconstitution of the detergent extract. Surprisingly, depletion of SSR was without effect on any of the assayed activities, indicating that SSR is either not required for translocation or is one of a family of functionally redundant components.

MeSH Terms
Amino Acid Sequence Animals Antibodies/isolation & purification Calcium-Binding Proteins Cattle Dogs Macromolecular Substances Membrane Glycoproteins/metabolism Microsomes/metabolism Molecular Sequence Data Molecular Weight Oligopeptides/chemical synthesis,immunology Pancreas/metabolism Peptide Chain Elongation, Translational Prolactin/genetics Protein Biosynthesis Protein Precursors/genetics Protein Processing, Post-Translational RNA, Messenger/metabolism Receptors, Cell Surface/analysis,metabolism Receptors, Cytoplasmic and Nuclear Receptors, Peptide Ribosomes/metabolism Transcription, Genetic
Chemicals
Antibodies Calcium-Binding Proteins Macromolecular Substances Membrane Glycoproteins Oligopeptides Protein Precursors RNA, Messenger Receptors, Cell Surface Receptors, Cytoplasmic and Nuclear Receptors, Peptide signal peptide receptor signal sequence receptor preprolactin Prolactin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Migliaccio G
Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York 10021-6399.
Nicchitta C V
Blobel G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.117.1.15
Published
1992-04-00
Pages
15-25
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289408
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.117.1.15 PMC Full Text View journal details
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