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PMID: 7828587 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The identity of the base following the stop codon determines the efficiency of in vivo translational termination in Escherichia coli.

The EMBO journal ·Vol. 14 ·No. 1 ·1995-01-03 ·Pages 151-8

Poole ES, Brown CM, Tate WP

Abstract

A statistical analysis of > 2000 Escherichia coli genes suggested that the base following the translational stop codon might be an important feature of the signal for termination. The strengths of each of 12 possible 'four base stop signals' (UAAN, UGAN and UAGN) were tested in an in vivo termination assay that measured termination efficiency by its direct competition with frameshifting. Termination efficiencies varied significantly depending on both the stop codon and the fourth base, ranging from 80 (UAAU) to 7% (UGAC). For both the UAAN and UGAN series, the fourth base hierarchy was U > G > A approximately C. UAG stop codons, which are used rarely in E. coli, showed efficiencies comparable with UAAN and UGAN, but differed in that the hierarchy of the fourth base was G > U approximately A > C. The rate of release factor selection varied 30-fold at UGAN stop signals, and 10-fold for both the UAAN and UAGN series; it correlated well with the frequency with which the different UAAN and UGAN signals are found at natural termination sites. The results suggest that the identity of the base following the stop codon determines the efficiency of translational termination in E. coli. They also provide a rationale for the use of the strong UAAU signal in highly expressed genes and for the occurrence of the weaker UGAC signal at several recording sites.

MeSH Terms
Base Composition Base Sequence Codon, Terminator/genetics Escherichia coli/genetics Genes, Bacterial Molecular Sequence Data Peptide Chain Termination, Translational/genetics Reading Frames/genetics Structure-Activity Relationship
Chemicals
Codon, Terminator
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Poole E S
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Brown C M
Tate W P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-01-03
Pages
151-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC398062
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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