Home LiteratureArticle Details
PMID: 6419024 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A temperature-sensitive mutant of Escherichia coli that shows enhanced misreading of UAG/A and increased efficiency for some tRNA nonsense suppressors.

Molecular & general genetics : MGG ·Vol. 193 ·No. 1 ·1984-00-00 ·Pages 38-45

Rydén SM, Isaksson LA

Abstract

A spontaneous mutant was isolated that harbors a weak suppressing activity towards a UAG mutation, together with an inability to grow at 43 degrees C in rich medium. The mutation is shown to be associated with an increased misreading of UAG at certain codon contexts and UAA. UGA, missense or frameshift mutations do not appear to be misread to a similar extent. The mutation gives an increased efficiency to several amber tRNA suppressors without increasing their ambiguity towards UAA. The ochre suppressors SuB and Su5 are stimulated in their reading of both UAG and UAA with preference for UAG. An opal suppressor is not affected. The effect of the mutation on the efficiency of amber and ochre suppressors is dependent on the codon context of the nonsense codon. The mutated gene (uar) has been mapped and found to be recessive both with respect to suppressor-enhancing ability as well as for temperature sensitivity. The phenotype is partly suppressed by the ochre suppressor SuC. It is suggested that uar codes for a protein, which is involved in translational termination at UAG and UAA stop codons.

MeSH Terms
Chromosomes, Bacterial Codon Crosses, Genetic Escherichia coli/genetics Genes, Bacterial Mutation RNA, Messenger RNA, Transfer/genetics Species Specificity Suppression, Genetic Temperature Transduction, Genetic beta-Glucosidase/genetics
Chemicals
Codon RNA, Messenger RNA, Transfer beta-Glucosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rydén S M
Isaksson L A
References (37)
37 references, click to expand
  1. High efficiency temperature-sensitive amber suppressor strains of Escherichia coli K12: isolation of strains with suppressor-enhancing mutations.
    Mol Gen Genet. 1980;177(4):545-52 PMID: 6991863
  2. Compilation of tRNA sequences.
    Nucleic Acids Res. 1982 Jan 22;10(2):r1-55 PMID: 7063409
  3. More mutant tyrosine transfer ribonucleic acids.
    J Mol Biol. 1970 Nov 28;54(1):1-14 PMID: 4923867
  4. Adjacent effect on suppression efficiency. II. Study on ochre and amber mutants of T4 phage lysozyme.
    Mol Gen Genet. 1970;106(3):208-12 PMID: 5480467
  5. Genetic studies on temperature sensitive nonsense suppression.
    Mol Gen Genet. 1970;106(4):362-70 PMID: 4921209
  6. A mutation that increases the activity of nonsense suppressors in Escherichia coli.
    Mol Gen Genet. 1978 Feb 16;159(2):161-9 PMID: 345090
  7. The influence of the reading context upon the suppression of nonsense codons.
    Mol Gen Genet. 1977 Mar 7;151(2):137-49 PMID: 327262
  8. Effect of different mutations in ribosomal protein S5 of Escherichia coli on translational fidelity.
    Mol Gen Genet. 1975 Sep 29;140(2):91-100 PMID: 1105158
  9. Tryptophan transfer RNA as the UGA suppressor.
    J Mol Biol. 1971 Jun 14;58(2):439-58 PMID: 4933412
  10. The influence of the reading context upon the suppression of nonsense codons.
    Mol Gen Genet. 1969 Oct 13;105(2):125-30 PMID: 5367414
  11. Linkage map of Escherichia coli K-12, edition 7.
    Microbiol Rev. 1983 Jun;47(2):180-230 PMID: 6348505
  12. Ribosomal discrimination of tRNAs.
    Nat New Biol. 1971 Dec 29;234(52):261-4 PMID: 4943838
  13. Analysis of rpsD mutations in Escherichia coli. I. Comparison of mutants with various alterations in ribosomal protein S4.
    Mol Gen Genet. 1979 Feb 1;169(3):251-7 PMID: 372747
  14. The influence of codon context on genetic code translation.
    Nature. 1980 Jul 10;286(5769):123-7 PMID: 7402305
  15. Cooperative control of translational fidelity by ribosomal proteins in Escherichia coli. III. A ram mutation in the structural gene for protein S5 (rpx E).
    Mol Gen Genet. 1976 Feb 27;144(1):59-62 PMID: 772415
  16. Coding properties of an ochre-suppressing derivative of Escherichia coli tRNAITyr.
    J Mol Biol. 1977 May 25;112(3):453-70 PMID: 327078
  17. Isolation and characterization of context mutations affecting the suppressibility of nonsense mutations.
    Mol Gen Genet. 1980;177(4):615-27 PMID: 6991868
  18. Isolation and characterization of a temperature-sensitive amber suppressor mutant of Escherichia coli K12.
    Mol Gen Genet. 1973;123(1):77-88 PMID: 4580085
  19. Genetic studies of the lac repressor. III. Additional correlation of mutational sites with specific amino acid residues.
    J Mol Biol. 1977 Dec 15;117(3):525-67 PMID: 609095
  20. Amino acid acceptor stem of E. coli suppressor tRNA tyr is a site of synthetase recognition.
    Nat New Biol. 1973 Aug 29;244(139):261-4 PMID: 4580701
  21. Context effects on nonsense codon suppression in Escherichia coli.
    Genetics. 1978 Feb;88(2):201-19 PMID: 346437
  22. Electrophoretic and immunological studies on ribosomal proteins of 100 Escherichia coli revertants from streptomycin dependence.
    Mol Gen Genet. 1973 Dec 14;127(1):1-18 PMID: 4589343
  23. Analysis of rpsD mutations in Escherichia coli. II. Physiology of some representative mutants.
    Mol Gen Genet. 1979 Feb 1;169(3):259-69 PMID: 372748
  24. A model for three-point analysis of random general transduction.
    Genetics. 1966 Aug;54(2):405-10 PMID: 5338813
  25. Effect of neighboring nucleotide sequences on suppression efficiency in amber mutants of T4 phage lysozyme.
    Mol Gen Genet. 1976 Nov 24;149(1):1-4 PMID: 1012265
  26. Mutant tyrosine tRNA of altered amino acid specificity.
    FEBS Lett. 1972 Apr 15;22(1):144-148 PMID: 11946582
  27. The influence of the reading context upon the suppression of nonsense codons. 3.
    Cold Spring Harb Symp Quant Biol. 1969;34:513-20 PMID: 5266175
  28. A functional requirement for modification of the wobble nucleotide in tha anticodon of a T4 suppressor tRNA.
    Cell. 1976 Nov;9(3):449-63 PMID: 791507
  29. High efficiency temperature-sensitive amber suppressor strains of Escherichia coli K12: construction and characterization of recombinant strains with suppressor-enhancing mutations.
    Mol Gen Genet. 1980;178(2):293-9 PMID: 6771486
  30. A temperature-sensitive suppressor enabling the manipulation of the level of individual proteins in intact cells.
    Cell. 1976 Feb;7(2):205-12 PMID: 782718
  31. Translation rates and misreading characteristics of rpsD mutants in Escherichia coli.
    Mol Gen Genet. 1982;187(3):467-72 PMID: 6757661
  32. Phage P1 mutants with altered transducing abilities for Escherichia coli.
    Virology. 1974 Jun;59(2):532-44 PMID: 4598709
  33. Letter: Mutant tyrosine transfer ribonucleic acids of Escherichia coli: construction by recombination of a double mutant A1G82 chargeable with glutamine.
    J Mol Biol. 1974 May 5;85(1):187-92 PMID: 4599146
  34. Effects of surrounding sequence on the suppression of nonsense codons.
    J Mol Biol. 1983 Feb 15;164(1):59-71 PMID: 6188840
  35. Mischarging in mutant tyrosine transfer RNAs.
    FEBS Lett. 1972 Apr 15;22(1):149-155 PMID: 11946583
  36. Alteration of a 30S ribosomal protein accompanying the ram mutation in Escherichia coli.
    Proc Natl Acad Sci U S A. 1971 Sep;68(9):2263-7 PMID: 4943794
  37. Correlation of nonsense sites in the lacI gene with specific codons in the nucleotide sequence.
    Nature. 1978 Aug 24;274(5673):770-5 PMID: 355892
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00327411
Published
1984-00-00
Pages
38-45
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00327411
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2