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PMID: 772415 Published · ppublish English Journal Article

Cooperative control of translational fidelity by ribosomal proteins in Escherichia coli. III. A ram mutation in the structural gene for protein S5 (rpx E).

Molecular & general genetics : MGG ·Vol. 144 ·No. 1 ·1976-02-27 ·Pages 59-62

Cabezón T, Herzog A, De Wilde M, Villarroel R, Bollen A

Abstract

The effect on translational fidelity of a particular mutation in the gene coding for protein S5(rpxE) has been investigated. This mutation has the opposite effect of a restrictive strA mutation; in vivo, it relieves the restriction imposed by strA on the suppression of T4 nonsense mutants and results in hypersensitivity to streptomycin; in vitro, the presence of the altered S5 protein in 30S ribosomes results in increased intrinsic misreading. It is concluded that this mutation, ramC319, acts as a ribosomal ambiguity mutation similar to certain mutations of protein S4 (ramA).

MeSH Terms
Bacterial Proteins/biosynthesis Drug Resistance, Microbial Escherichia coli/drug effects,metabolism Genes Mutation Phenotype Protein Biosynthesis Ribosomal Proteins/biosynthesis Streptomycin/pharmacology
Chemicals
Bacterial Proteins Ribosomal Proteins Streptomycin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cabezón T
Herzog A
De Wilde M
Villarroel R
Bollen A
References (23)
23 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00277305
Published
1976-02-27
Pages
59-62
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00277305
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