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PMID: 781621 Published · ppublish English Journal Article

The involvement of the anticodon adjacent modified nucleoside N-(9-(BETA-D-ribofuranosyl) purine-6-ylcarbamoyl)-threonine in the biological function of E. coli tRNAile.

Nucleic acids research ·Vol. 3 ·No. 5 ·1976-05-00 ·Pages 1185-201

Miller JP, Hussain Z, Schweizer MP

Abstract

tRNAile was isolated from E. coli Cp 79 (leu-, arg-, thr-, his-, thiamin-, RCrel) which had been grown on a sub-optimal concentration of thr and was found to contain an average of 50% less N-[9-(beta-D-ribofuranosyl)- purin-6-ylcarbamoyl]threonine, t6Ado, than tRNAile from cells grown on an optimum concentration of thr and containing a normal complement of t6Ado. The two tRNA's were identical in their ability to be aminoacylated, to accept the 3'-terminal dinucleotide, and to form an ile-tRNAile-Tu-GTP complex. In contrast, the t6Ado-deficient-tRNA was significantly less efficient in binding to ribosomes compared to the normal tRNA. This difference was seen in the binding of deacylated tRNA and in the nonenzymatic and enzymatic binding of ile-tRNA, all in response to poly AUC. The t6Ado-deficient ile-tRNA demonstrated no binding at Mg2+ concentrations less than or equal to 10 mM, while the normal ile-tRNA bound at low Mg2+ concentrations. Tetracycline had the same effect on the normal as on the t6Ado-deficient ile-tRNA binding. As a control, the binding of phe-tRNA (which does not contain t6Ado) from normal and thr-starved cells in response to poly U was identical. It was concluded that t6Ado is required for proper codon-anticodon interaction.

MeSH Terms
Amino Acyl-tRNA Synthetases/metabolism Anticodon Escherichia coli/drug effects,metabolism Isoleucine Kinetics Magnesium/pharmacology Nucleotidyltransferases/metabolism RNA, Transfer/metabolism RNA, Viral/metabolism Ribonucleosides/analysis Ribosomes/drug effects,metabolism Tetracycline/pharmacology Threonine/analysis
Chemicals
Anticodon RNA, Viral Ribonucleosides Isoleucine Threonine RNA, Transfer Nucleotidyltransferases Amino Acyl-tRNA Synthetases Tetracycline Magnesium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Miller J P
Hussain Z
Schweizer M P
References (41)
41 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/3.5.1185
Published
1976-05-00
Pages
1185-201
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342979
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/3.5.1185 PMC Full Text View journal details
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