The enzymatic synthesis of N-(purin-6-ylcarbamoyl)threonine, an anticodon-adjacent base in transfer ribonucleic acid.

Adenosine Triphosphate/pharmacology Amino Acyl-tRNA Synthetases/antagonists & inhibitors,isolation & purification,metabolism Anticodon/metabolism Bicarbonates/metabolism,pharmacology Carbon Radioisotopes Chromatography, DEAE-Cellulose Electrophoresis, Paper Electrophoresis, Polyacrylamide Gel Enzyme Activation/drug effects Escherichia coli/drug effects,metabolism Glycine/pharmacology Hydrogen-Ion Concentration Hydrolysis Kinetics Magnesium/pharmacology Molecular Weight Mutation Purines/biosynthesis RNA, Bacterial/isolation & purification,metabolism RNA, Transfer/isolation & purification,metabolism Threonine/metabolism,pharmacology Tritium

Anticodon Bicarbonates Carbon Radioisotopes Purines RNA, Bacterial Tritium Threonine Adenosine Triphosphate RNA, Transfer Amino Acyl-tRNA Synthetases Magnesium Glycine