Home LiteratureArticle Details
PMID: 7809114 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Pax-3 contains domains for transcription activation and transcription inhibition.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 26 ·1994-12-20 ·Pages 12745-9

Chalepakis G, Jones FS, Edelman GM, Gruss P

Abstract

Pax-3 is a member of the Pax family of transcription factors involved in transcriptional control events during embryonic development. Here we report a functional dissection of the Pax-3 protein and describe the protein domains which are responsible for different activities. A transcription inhibition activity is located in the first 90 N-terminal amino acids and includes part of the paired domain. Furthermore, the C terminus of Pax-3 is able to confer transcriptional activation of basal promoters. Pax-3 can utilize both transcription modulating functions and activates transcription over a narrow range of protein concentration in the presence of promoter elements containing functional binding sites.

Related Genes
Pax-3
MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites Cell Adhesion Molecules, Neuronal/genetics DNA Mutational Analysis DNA-Binding Proteins/chemistry,metabolism Gene Expression Regulation Homeodomain Proteins/chemistry In Vitro Techniques Mice Molecular Sequence Data PAX3 Transcription Factor Paired Box Transcription Factors Promoter Regions, Genetic Structure-Activity Relationship Transcription Factors/chemistry Transcription, Genetic Transcriptional Activation
Chemicals
Cell Adhesion Molecules, Neuronal DNA-Binding Proteins Homeodomain Proteins PAX3 Transcription Factor Paired Box Transcription Factors Transcription Factors Pax3 protein, mouse
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chalepakis G
Department of Molecular Cell Biology, Max Planck Institute for Biophysical Chemistry, Göttingen, Federal Republic of Germany.
Jones F S
Edelman G M
Gruss P
References (27)
27 references, click to expand
  1. Cell adhesion molecules in the regulation of animal form and tissue pattern.
    Annu Rev Cell Biol. 1986;2:81-116 PMID: 3548776
  2. Molecular characterization of a deletion encompassing the splotch mutation on mouse chromosome 1.
    Genomics. 1991 May;10(1):89-93 PMID: 2045114
  3. Splotch (Sp2H), a mutation affecting development of the mouse neural tube, shows a deletion within the paired homeodomain of Pax-3.
    Cell. 1991 Nov 15;67(4):767-74 PMID: 1682057
  4. Waardenburg's syndrome patients have mutations in the human homologue of the Pax-3 paired box gene.
    Nature. 1992 Feb 13;355(6361):635-6 PMID: 1347148
  5. An exonic mutation in the HuP2 paired domain gene causes Waardenburg's syndrome.
    Nature. 1992 Feb 13;355(6361):637-8 PMID: 1347149
  6. Cell adhesion molecules as targets for Hox genes: neural cell adhesion molecule promoter activity is modulated by cotransfection with Hox-2.5 and -2.4.
    Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2086-90 PMID: 1347944
  7. N-CAM alterations in splotch neural tube defect mouse embryos.
    Development. 1991 Nov;113(3):1049-58 PMID: 1821845
  8. A mutation within intron 3 of the Pax-3 gene produces aberrantly spliced mRNA transcripts in the splotch (Sp) mouse mutant.
    Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):532-6 PMID: 8421686
  9. Mutations in the PAX3 gene causing Waardenburg syndrome type 1 and type 2.
    Nat Genet. 1993 Jan;3(1):26-30 PMID: 8490648
  10. A frameshift mutation in the HuP2 paired domain of the probable human homolog of murine Pax-3 is responsible for Waardenburg syndrome type 1 in an Indonesian family.
    Hum Mol Genet. 1992 Jul;1(4):243-7 PMID: 1303193
  11. Cloning and characterization of the inversion breakpoint at chromosome 2q35 in a patient with Waardenburg syndrome type I.
    Hum Mol Genet. 1992 Aug;1(5):315-7 PMID: 1303207
  12. Rearrangement of the PAX3 paired box gene in the paediatric solid tumour alveolar rhabdomyosarcoma.
    Nat Genet. 1993 Feb;3(2):113-7 PMID: 8098985
  13. The oncogenic potential of Pax genes.
    EMBO J. 1993 Jun;12(6):2361-7 PMID: 8099544
  14. Analysis of the Pax-3 gene in the mouse mutant splotch.
    Genomics. 1993 Aug;17(2):355-63 PMID: 8406486
  15. Fusion of a fork head domain gene to PAX3 in the solid tumour alveolar rhabdomyosarcoma.
    Nat Genet. 1993 Nov;5(3):230-5 PMID: 8275086
  16. Molecular basis of splotch and Waardenburg Pax-3 mutations.
    Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3685-9 PMID: 7909605
  17. Pax-3-DNA interaction: flexibility in the DNA binding and induction of DNA conformational changes by paired domains.
    Nucleic Acids Res. 1994 Aug 11;22(15):3131-7 PMID: 8065927
  18. The yeast UASG is a transcriptional enhancer in human HeLa cells in the presence of the GAL4 trans-activator.
    Cell. 1988 Jan 29;52(2):169-78 PMID: 2830022
  19. How eukaryotic transcriptional activators work.
    Nature. 1988 Oct 20;335(6192):683-9 PMID: 3050531
  20. Conservation of the paired domain in metazoans and its structure in three isolated human genes.
    EMBO J. 1989 Apr;8(4):1183-90 PMID: 2501086
  21. The proline-rich transcriptional activator of CTF/NF-I is distinct from the replication and DNA binding domain.
    Cell. 1989 Aug 25;58(4):741-53 PMID: 2504497
  22. A vector for expressing GAL4(1-147) fusions in mammalian cells.
    Nucleic Acids Res. 1989 Sep 25;17(18):7539 PMID: 2798115
  23. Differential transcriptional activation by Oct-1 and Oct-2: interdependent activation domains induce Oct-2 phosphorylation.
    Cell. 1990 Feb 9;60(3):375-86 PMID: 2302733
  24. Identification of positive and negative regulatory elements governing cell-type-specific expression of the neural cell adhesion molecule gene.
    Mol Cell Biol. 1990 May;10(5):1959-68 PMID: 2325642
  25. Transcriptional repression in eukaryotes.
    Trends Genet. 1990 Jun;6(6):192-7 PMID: 2196723
  26. Pax-3, a novel murine DNA binding protein expressed during early neurogenesis.
    EMBO J. 1991 May;10(5):1135-47 PMID: 2022185
  27. The molecular basis of the undulated/Pax-1 mutation.
    Cell. 1991 Sep 6;66(5):873-84 PMID: 1889089
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.26.12745
Published
1994-12-20
Pages
12745-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC45516
Subset
IM
Grants
NIA NIH HHS · AG09326 · United States
NICHD NIH HHS · HD09635 · United States
External Links
PubMed source DOI: 10.1073/pnas.91.26.12745 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2