Isolation, sequencing, and mutagenesis of the gene encoding NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) from Paracoccus denitrificans, in which GD-FALDH is essential for methylotrophic growth.

Journal of bacteriology ·Vol. 177 ·No. 1 ·1995-01-00 ·Pages 247-51

Ras J, Van Ophem PW, Reijnders WN, Van Spanning RJ, Duine JA, Stouthamer AH, Harms N

Abstract

NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) of Paracoccus denitrificans has been purified as a tetramer with a relative molecular mass of 150 kDa. The gene encoding GD-FALDH (flhA) has been isolated, sequenced, and mutated by insertion of a kanamycin resistance gene. The mutant strain is not able to grow on methanol, methylamine, or choline, while heterotrophic growth is not influenced by the mutation. This finding indicates that GD-FALDH of P. denitrificans is essential for the oxidation of formaldehyde produced during methylotrophic growth.

MeSH Terms

Aldehyde Oxidoreductases/genetics,isolation & purification Amino Acid Sequence Base Sequence Cloning, Molecular Formaldehyde/metabolism Genes, Bacterial/genetics Methanol/metabolism Molecular Sequence Data Mutagenesis Paracoccus denitrificans/genetics,growth & development,metabolism Sequence Analysis, DNA Sequence Homology, Amino Acid

Chemicals

Formaldehyde formaldehyde dehydrogenase (glutathione) Aldehyde Oxidoreductases Methanol

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Ras J

Department of Microbiology, Biological Laboratory, Vrije Universiteit, Amsterdam, The Netherlands.

Van Ophem P W

Reijnders W N

Van Spanning R J

Duine J A

Stouthamer A H

Harms N

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.177.1.247-251.1995

Published

1995-01-00

Pages

247-51

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC176581

Subset

Databases

GENBANK

L36327, U34346

External Links

PubMed source DOI: 10.1128/jb.177.1.247-251.1995 PMC Full Text View journal details

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Isolation, sequencing, and mutagenesis of the gene encoding NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) from Paracoccus denitrificans, in which GD-FALDH is essential for methylotrophic growth.

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