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PMID: 778849 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Morphogenesis of the bacterial division septum: a new class of septation-defective mutants.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 6 ·1976-06-00 ·Pages 1882-6

Weigand RA, Vinci KD, Rothfield LI

Abstract

A new class of mutants of Salmonella typhimurium (lkyD mutants) are described. The mutants are defective in morphogenesis of the division septum, and are characterized by a failure of the outer membrane to invaginate despite normal ingrowth of the cytoplasmic membrane and murein layers of the growing septum. The cell envelopes of the mutants show a significant decrease in the bound form of murein-lipoprotein and a corresponding increase in the free form of the lipoprotein. This suggests that the morphogenic defect may result from a defect in formation of covalent bonds between the free lipoprotein of the outer membrane and the murein of the nascent septum.

MeSH Terms

Amino Acids/analysis Bacterial Proteins/analysis,metabolism Cell Division Lipoproteins/analysis,metabolism Membranes/metabolism,ultrastructure Mutation Phenotype Salmonella typhimurium/growth & development,ultrastructure Solubility

Chemicals

Amino Acids Bacterial Proteins Lipoproteins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Weigand R A

Vinci K D

Rothfield L I

References (9)

9 references, click to expand

BIOSYNTHESIS OF BACTERIAL LIPOPOLYSACCHARIDE. II. INCORPORATION OF GLUCOSE AND GALACTOSE CATALYZED BY PARTICULATE AND SOLUBLE ENZYMES IN SALMONELLA.
J Biol Chem. 1964 Sep;239:2788-95 PMID: 14217875
Supramolecular structure of the rigid layer of the cell wall of Salmonella, Serratia, Proteus, and Pseudomonas fluorescens. Number of lipoprotein molecules in a membrane layer.
Biochemistry. 1970 Dec 22;9(26):5041-9 PMID: 4249403
The assembly of a structural lipoprotein in the envelope of Escherichia coli.
J Biol Chem. 1972 Dec 25;247(24):8154-9 PMID: 4565677
Identification of an outer membrane protein of Escherichia coli, with a role in the coordination of deoxyribonucleic acid replication and cell elongation.
J Bacteriol. 1975 Nov;124(2):918-29 PMID: 171254
Cell division in a chain-forming envA mutant of Escherichia coli K12. Fine structure of division sites and effects of EDTA, lysozyme and ampicillin.
Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(5):651-64 PMID: 4999789
Genetics of a chain-forming mutant of Escherichia coli. Transduction and dominance of the envA gene mediating increased penetration to some antibacterial agents.
Genet Res. 1970 Aug;16(1):63-78 PMID: 4922970
Chemical characterization, spatial distribution and function of a lipoprotein (murein-lipoprotein) of the E. coli cell wall. The specific effect of trypsin on the membrane structure.
Eur J Biochem. 1969 Oct;10(3):426-38 PMID: 4899922
Genetic and physiological classification of periplasmic-leaky mutants of Salmonella typhimurium.
J Bacteriol. 1976 Jan;125(1):340-5 PMID: 812862
Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system.
J Biol Chem. 1971 Oct 25;246(20):6328-34 PMID: 5127429

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.73.6.1882

Published

1976-06-00

Pages

1882-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC430411

Subset

External Links

PubMed source DOI: 10.1073/pnas.73.6.1882 PMC Full Text View journal details

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Morphogenesis of the bacterial division septum: a new class of septation-defective mutants.

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