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PMID: 812862 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Genetic and physiological classification of periplasmic-leaky mutants of Salmonella typhimurium.

Journal of bacteriology ·Vol. 125 ·No. 1 ·1976-01-00 ·Pages 340-5

Weigand RA, Rothfield LI

Abstract

Mutants of Salmonella typhimurium that leaked periplasmic proteins were isolated. Four classes of mutants were identified by their increased sensitivity to dyes, detergents, or antibiotics. Conjugation studies indicated that representatives of two classes mapped in the proA-galE region of the Salmonella chromosome and two in the cysI-argE region. According to their bacteriophage sensitivity pattern, all four of the mutant classes appear to retain the smooth lipopolysaccharide characteristic. One class of mutants has an abnormal cell envelope in which the outer membrane balloons away from the murein layer.

MeSH Terms
Acridines/pharmacology Bacterial Proteins/metabolism Cell Wall/ultrastructure Chromosome Mapping Conjugation, Genetic Cytoplasm/metabolism Deoxycholic Acid/pharmacology Drug Resistance, Microbial Edetic Acid/pharmacology Genotype Methylene Blue/pharmacology Mutation Phenotype Rifampin/pharmacology Salmonella typhimurium/drug effects,metabolism,ultrastructure Sodium Dodecyl Sulfate/pharmacology
Chemicals
Acridines Bacterial Proteins Deoxycholic Acid Sodium Dodecyl Sulfate Edetic Acid Methylene Blue Rifampin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Weigand R A
Rothfield L I
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20 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.125.1.340-345.1976
Published
1976-01-00
Pages
340-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC233368
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.125.1.340-345.1976 PMC Full Text View journal details
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