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PMID: 7774596 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Escherichia coli RecG and RecA proteins in R-loop formation.

The EMBO journal ·Vol. 14 ·No. 10 ·1995-05-15 ·Pages 2385-92

Hong X, Cadwell GW, Kogoma T

Abstract

Escherichia coli rnhA mutants devoid of RNase HI exhibit constitutive stable DNA replication, cSDR, which is thought to be initiated from R-loops stabilized in the absence of RNase HI. We found that a combination of an rnhA and a recG mutation is lethal to the cell. recG mutations that inactivate the helicase activity of RecG protein and inhibit reverse branch migration of Holliday junctions impart phenotypes resembling those of rnhA mutants. Thus, recG mutants display cSDR activity, and recG polA double mutants are inviable as are rnhA polA double mutants. These results suggest that the RecG helicase has a role in preventing R-loop formation. A model that R-loops are formed by assimilation of RNA transcripts into the duplex DNA is discussed. The model further postulates that RecA protein catalyzes this assimilation reaction and that RecG protein counteracts RecA in this reaction, resolves R-loops by its helicase activity, or does both.

Related Genes
polA recA recG rnhA
MeSH Terms
Bacterial Proteins/genetics,metabolism DNA Helicases/genetics,metabolism DNA Polymerase I/genetics DNA Repair DNA Replication/genetics DNA, Bacterial/biosynthesis DNA-Binding Proteins/genetics Escherichia coli/genetics Escherichia coli Proteins Genes, Bacterial/genetics Genes, Lethal/genetics Kanamycin Resistance/genetics Models, Genetic Mutation Nucleic Acid Conformation Nucleic Acid Heteroduplexes Rec A Recombinases/metabolism Recombination, Genetic Replication Origin Ribonuclease H/analysis,genetics SOS Response, Genetics Sequence Analysis, DNA
Chemicals
Bacterial Proteins DNA, Bacterial DNA-Binding Proteins DnaA protein, Bacteria Escherichia coli Proteins Nucleic Acid Heteroduplexes RecG protein, E coli Rec A Recombinases DNA Polymerase I Ribonuclease H ribonuclease HI DNA Helicases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hong X
Department of Cell Biology, University of New Mexico Health Sciences Center, Albuquerque 87131, USA.
Cadwell G W
Kogoma T
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53 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-05-15
Pages
2385-92
Language
English
Region
England
NLM ID
8208664
PMCID
PMC398347
Subset
IM
Grants
NIGMS NIH HHS · GM22092 · United States
External Links
PubMed source PMC Full Text View journal details
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