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PMID: 777046 Published · ppublish English Journal Article

Classification of smooth muscle autoantibodies detected by immunofluorescence.

Journal of clinical pathology ·Vol. 29 ·No. 5 ·1976-05-00 ·Pages 403-10

Bottazzo GF, Florin-Christensen A, Fairfax A, Swana G, Doniach D, Groeschel-Stewart U

Abstract

Three hundred and twelve sera containing antibodies to smooth muscle (SMA) wer analysed for the immunofluorescence patterns they produced in various tissues. A classification is described based on the three main appearances in rat kidney. Some sera, mainly of low titre, reacted only with vessel walls (SMA-V), some stained vessels and renal glomeruli (SMA-G) and high titre sera, mainly from patients with chronic active hepatitis stained vessels, glomeruli, and intracellular fibrils in renal tubules (SMA-T). Peripheral staining in hepatocytes or thyroid cells was not a regular feature. 41/43 polyclonal SMA-T and -G sera were absorbed out completely by actin, and this also removed the pericullular staining in liver and thyroid when present. High titre SMA-V antibodies could not be absorbed by actin, and the antigen remains to be identified.

MeSH Terms
Animals Autoantibodies/analysis,classification Cattle Fluorescent Antibody Technique Hepatitis/immunology Humans Kidney/immunology Kidney Glomerulus/immunology Kidney Tubules/immunology Liver/immunology Liver Diseases/immunology Mice Muscle Proteins/immunology Muscle, Smooth/immunology Muscles/immunology Rats T-Lymphocytes/immunology Thymus Gland/immunology Thyroid Gland/immunology
Chemicals
Autoantibodies Muscle Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Bottazzo G F
Florin-Christensen A
Fairfax A
Swana G
Doniach D
Groeschel-Stewart U
References (33)
33 references, click to expand
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Article Info
Journal
Journal of clinical pathology
Abbr.
J Clin Pathol
ISSN
0021-9746
DOI
10.1136/jcp.29.5.403
Published
1976-05-00
Pages
403-10
Language
English
Region
England
NLM ID
0376601
PMCID
PMC476077
Subset
IM
External Links
PubMed source DOI: 10.1136/jcp.29.5.403 PMC Full Text View journal details
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