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PMID: 7768869 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

ccrA1: a mutation in Streptomyces coelicolor that affects the control of catabolite repression.

Journal of bacteriology ·Vol. 177 ·No. 12 ·1995-06-00 ·Pages 3579-86

Ingram C, Delic I, Westpheling J

Abstract

The regulation of carbon utilization is of central importance in the gene expression pathways for both morphological development and antibiotic production in Streptomyces species. We report the identification and characterization of a mutation in Streptomyces coelicolor, ccrA1, that affects the expression of several catabolite-controlled promoters. ccrA1 mutants are altered in expression of galP1, the glucose-sensitive, galactose-dependent promoter of the galactose utilization operon; in expression of the glycerol utilization operon, which is glucose sensitive and glycerol dependent; and in expression of chi63, the glucose-sensitive chitin-dependent promoter of a gene involved in chitin utilization. ccrA1 has no effect on the expression of galP2, a promoter that directs constitutive transcription of the galE and galK genes. ccrA1 maps to a region of the S. coelicolor genome which distinguishes it from other mutations known to be involved in catabolite control. We suggest that ccrA1 identifies a gene whose product may be involved in the general regulation of carbon catabolite repression in this complex bacterium.

MeSH Terms
Chromosome Mapping Crosses, Genetic Down-Regulation/genetics Gene Expression Regulation, Bacterial/genetics Mutation Operon/genetics Promoter Regions, Genetic Streptomyces/genetics,metabolism
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ingram C
Molecular Biology Graduate Program, University of Pennsylvania, Philadelphia, USA.
Delic I
Westpheling J
References (41)
41 references, click to expand
  1. Cyclic 3'5-AMP: stimulation of beta-galactosidase and tryptophanase induction in E. coli.
    Biochem Biophys Res Commun. 1968 Mar 27;30(6):656-64 PMID: 4966929
  2. At least three different RNA polymerase holoenzymes direct transcription of the agarase gene (dagA) of Streptomyces coelicolor A3(2).
    Cell. 1988 Feb 26;52(4):599-607 PMID: 3342448
  3. Cyclic adenosine monophosphate in bacteria.
    Science. 1970 Jul 24;169(3943):339-44 PMID: 4317896
  4. Characterization of temperate actinophage phi C31 isolated from Streptomyces coelicolor A3(2).
    J Virol. 1972 Feb;9(2):258-62 PMID: 5014931
  5. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  6. Cyclic adenosine 5'-monophosphate in Escherichia coli.
    Bacteriol Rev. 1976 Sep;40(3):527-51 PMID: 186018
  7. A new extragenic suppressor of cya mutation. Mutant cyclic AMP receptor protein with an increased affinity for cyclic AMP.
    J Biochem. 1978 Jun;83(6):1615-23 PMID: 209015
  8. Suppression of defects in cyclic adenosine 3',5'-monophosphate metabolism in Escherichia coli.
    J Bacteriol. 1980 Oct;144(1):205-9 PMID: 6252191
  9. Catabolite repression in Escherichia coli mutants lacking cyclic AMP receptor protein.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5799-801 PMID: 6255466
  10. Cloning of antibiotic resistance and nutritional genes in streptomycetes.
    J Bacteriol. 1982 Aug;151(2):668-77 PMID: 6284706
  11. Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples.
    J Biol Chem. 1982 Aug 10;257(15):8569-72 PMID: 7096324
  12. Chromogenic identification of genetic regulatory signals in Bacillus subtilis based on expression of a cloned Pseudomonas gene.
    Proc Natl Acad Sci U S A. 1983 Feb;80(4):1101-5 PMID: 6405380
  13. Compilation and analysis of Escherichia coli promoter DNA sequences.
    Nucleic Acids Res. 1983 Apr 25;11(8):2237-55 PMID: 6344016
  14. Glycerol catabolic enzymes and their regulation in wild-type and mutant strains of Streptomyces coelicolor A3(2).
    J Gen Microbiol. 1983 May;129(5):1403-13 PMID: 6619799
  15. The glycerol utilization operon of Streptomyces coelicolor: genetic mapping of gyl mutations and the analysis of cloned gylDNA.
    Mol Gen Genet. 1984;193(1):119-28 PMID: 6318046
  16. Genetic mapping, cloning and physiological aspects of the glucose kinase gene of Streptomyces coelicolor.
    Mol Gen Genet. 1984;196(3):501-7 PMID: 6094978
  17. Transcription of the Escherichia coli adenylate cyclase gene is negatively regulated by cAMP-cAMP receptor protein.
    J Biol Chem. 1985 Mar 10;260(5):3063-70 PMID: 2982847
  18. The Streptomyces plasmid SCP2*: its functional analysis and development into useful cloning vectors.
    Gene. 1985;35(3):223-35 PMID: 2995202
  19. Evidence for negative control of cya transcription by cAMP and cAMP receptor protein in intact Escherichia coli cells.
    J Biol Chem. 1985 Nov 25;260(27):14838-43 PMID: 2414300
  20. Characterization of Streptomyces promoter sequences using the Escherichia coli galactokinase gene.
    Gene. 1985;40(2-3):191-201 PMID: 3913624
  21. Two promoters, one inducible and one constitutive, control transcription of the Streptomyces lividans galactose operon.
    Proc Natl Acad Sci U S A. 1987 Apr;84(8):2130-4 PMID: 3031664
  22. New loci required for Streptomyces coelicolor morphological and physiological differentiation.
    J Bacteriol. 1988 Mar;170(3):1168-74 PMID: 3343216
  23. Cloning and transcription analysis of the entire glycerol utilization (gylABX) operon of Streptomyces coelicolor A3(2) and identification of a closely associated transcription unit.
    Mol Gen Genet. 1988 Jan;211(1):129-37 PMID: 2449598
  24. Cloning, characterization and regulation of an alpha-amylase gene from Streptomyces limosus.
    Mol Microbiol. 1988 Mar;2(2):197-208 PMID: 3260002
  25. Selection of DNA binding sites by regulatory proteins. II. The binding specificity of cyclic AMP receptor protein to recognition sites.
    J Mol Biol. 1988 Apr 20;200(4):709-23 PMID: 3045325
  26. Structure and regulation of controlling sequences for the Streptomyces coelicolor glycerol operon.
    J Mol Biol. 1988 Dec 5;204(3):569-80 PMID: 3225846
  27. Cyclic-AMP-dependent switch in initiation of transcription from the two promoters of the Escherichia coli gal operon: identification and assay of 5'-triphosphate ends of mRNA by GTP:RNA guanyltransferase.
    J Bacteriol. 1989 Mar;171(3):1623-30 PMID: 2537823
  28. xylE functions as an efficient reporter gene in Streptomyces spp.: use for the study of galP1, a catabolite-controlled promoter.
    J Bacteriol. 1989 Dec;171(12):6617-24 PMID: 2592344
  29. Genetic organization and regulation of the xylose degradation genes in Streptomyces rubiginosus.
    J Bacteriol. 1991 Nov;173(21):6849-58 PMID: 1657868
  30. Direct repeat sequences are implicated in the regulation of two Streptomyces chitinase promoters that are subject to carbon catabolite control.
    Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1885-9 PMID: 1542688
  31. Cloning and high-level expression of chitinase-encoding gene of Streptomyces plicatus.
    Gene. 1992 Feb 1;111(1):69-76 PMID: 1532161
  32. Cyclic AMP in prokaryotes.
    Microbiol Rev. 1992 Mar;56(1):100-22 PMID: 1315922
  33. The glucose kinase gene of Streptomyces coelicolor A3(2): its nucleotide sequence, transcriptional analysis and role in glucose repression.
    Mol Microbiol. 1992 Oct;6(19):2833-44 PMID: 1435260
  34. Identification of a complex operator for galP1, the glucose-sensitive, galactose-dependent promoter of the Streptomyces galactose operon.
    J Bacteriol. 1993 Mar;175(5):1213-20 PMID: 7680340
  35. Transcription activation at Class I CAP-dependent promoters.
    Mol Microbiol. 1993 May;8(5):797-802 PMID: 8394979
  36. The glucose kinase gene of Streptomyces coelicolor is not required for glucose repression of the chi63 promoter.
    J Bacteriol. 1995 Jun;177(12):3587-8 PMID: 7768870
  37. The agarase gene (dagA) of Streptomyces coelicolor A3(2): nucleotide sequence and transcriptional analysis.
    Mol Gen Genet. 1987 Aug;209(1):101-9 PMID: 17165236
  38. DNA deletions in spontaneous chloramphenicol-sensitive mutants of Streptomyces coelicolor A 3(2) and Streptomyces lividans 66.
    Mol Gen Genet. 1987 May;207(2-3):499-502 PMID: 3475527
  39. Gene organization and structure of the Streptomyces lividans gal operon.
    J Bacteriol. 1988 Jan;170(1):203-12 PMID: 3335481
  40. Cloning and expression of a Streptomyces plicatus chitinase (chitinase-63) in Escherichia coli.
    J Biol Chem. 1988 Jan 5;263(1):443-7 PMID: 3275646
  41. Pleiotropic deficiency of carbohydrate utilization in an adenyl cyclase deficient mutant of Escherichia coli.
    Biochem Biophys Res Commun. 1969 Sep 24;37(1):151-7 PMID: 4310317
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.12.3579-3586.1995
Published
1995-06-00
Pages
3579-86
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177065
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.177.12.3579-3586.1995 PMC Full Text View journal details
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