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PMID: 7761470 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The su(Hw) protein bound to gypsy sequences in one chromosome can repress enhancer-promoter interactions in the paired gene located in the other homolog.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 11 ·1995-05-23 ·Pages 5184-8

Georgiev PG, Corces VG

Abstract

The suppressor of Hairy-wing [su(Hw)] protein exerts a polar effect on gene expression by repressing the function of transcriptional enhancers located distally from the promoter with respect to the location of su(Hw) binding sequences. The directionality of this effect suggests that the su(Hw) protein specifically interferes with the basic mechanism of enhancer action. Moreover, mutations in modifier of mdg4 [mod(mdg4)] result in the repression of expression of a gene when the su(Hw) protein is bound to sequences in the copy of this gene located in the homologous chromosome. This effect is dependent on the presence of the su(Hw) binding region from the gypsy retrotransposon in at least one of the chromosomes and is enhanced by the presence of additional gypsy sequences in the other homology. This phenomenon is inhibited by chromosomal rearrangements that disrupt pairing, suggesting that close apposition between the two copies of the affected gene is important for trans repression of transcription. These results indicate that, in the absence of mod-(mdg4) product, the su(Hw) protein present in one chromosome can act in trans and inactivate enhancers located in the other homolog.

Related Genes

mdg4 mod su(Hw)

MeSH Terms

Alleles Animals Binding Sites Chromosome Mapping DNA-Binding Proteins/metabolism Drosophila Proteins Drosophila melanogaster/genetics Enhancer Elements, Genetic Female Gene Expression Genes, Insect Genotype Leucine Zippers Male Mutation Nuclear Proteins/metabolism Promoter Regions, Genetic Repressor Proteins Transcription, Genetic

Chemicals

DNA-Binding Proteins Drosophila Proteins Nuclear Proteins Repressor Proteins su(Hw) protein, Drosophila

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Georgiev P G

Institute of Gene Biology, Russian Academy of Sciences, Moscow.

Corces V G

References (25)

25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.92.11.5184

Published

1995-05-23

Pages

5184-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC41873

Subset

Grants

NIGMS NIH HHS · GM-35463 · United States

External Links

PubMed source DOI: 10.1073/pnas.92.11.5184 PMC Full Text View journal details

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The su(Hw) protein bound to gypsy sequences in one chromosome can repress enhancer-promoter interactions in the paired gene located in the other homolog.

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