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PMID: 7761468 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Ordered restriction endonuclease maps of yeast artificial chromosomes created by optical mapping on surfaces.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 11 ·1995-05-23 ·Pages 5164-8

Cai W, Aburatani H, Stanton VP, Housman DE, Wang YK, Schwartz DC

Abstract

We have developed a surface mounting technology for the rapid construction of ordered restriction maps from individual DNA molecules. Optical restriction maps constructed from yeast artificial chromosome DNA molecules mounted on specially derivatized glass surfaces are accurate and reproducible, and the technology is amenable to automation. The mounting procedures described here should also be useful for fluorescence in situ hybridization studies. We believe these improvements to optical mapping will further stimulate the development of nonelectrophoretic approaches to genome analysis.

MeSH Terms

Automation Chromosomes, Artificial, Yeast DNA, Fungal/chemistry,genetics,isolation & purification Microscopy, Fluorescence Molecular Weight Restriction Mapping Saccharomyces cerevisiae/genetics

Chemicals

DNA, Fungal

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Cai W

W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, NY 10003, USA.

Aburatani H

Stanton V P

Housman D E

Wang Y K

Schwartz D C

References (13)

13 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.92.11.5164

Published

1995-05-23

Pages

5164-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC41869

Subset

Grants

NHGRI NIH HHS · HG00225-02 · United States

NHGRI NIH HHS · HG00299 · United States

NHLBI NIH HHS · HL41484 · United States

External Links

PubMed source DOI: 10.1073/pnas.92.11.5164 PMC Full Text View journal details

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