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PMID: 7760829 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning of murine Stat6 and human Stat6, Stat proteins that are tyrosine phosphorylated in responses to IL-4 and IL-3 but are not required for mitogenesis.

Molecular and cellular biology ·Vol. 15 ·No. 6 ·1995-06-00 ·Pages 3336-43

Quelle FW, Shimoda K, Thierfelder W, Fischer C, Kim A, Ruben SM, Cleveland JL, Pierce JH, Keegan AD, Nelms K

Abstract

By searching a database of expressed sequences, we identified a member of the signal transducers and activators of transcription (Stat) family of proteins. Human and murine full-length cDNA clones were obtained and sequenced. The sequence of the human cDNA was identical to the recently published sequence for interleukin-4 (IL-4)-Stat (J. Hou, U. Schindler, W.J. Henzel, T.C. Ho, M. Brasseur, and S. L. McKnight, Science 265:1701-1706, 1994), while the murine Stat6 amino acid and nucleotide sequences were 83 and 84% identical to the human sequences, respectively. Using Stat6-specific antiserum, we demonstrated that Stat6 is rapidly tyrosine phosphorylated following stimulation of appropriate cell lines with IL-4 or IL-3 but is not detectably phosphorylated following stimulation with IL-2, IL-12, or erythropoietin. In contrast, IL-2, IL-3, and erythropoietin induce the tyrosine phosphorylation of Stat5 while IL-12 uniquely induces the tyrosine phosphorylation of Stat4. Inducible tyrosine phosphorylation of Stat6 requires the membrane-distal region of the IL-4 receptor alpha chain. This region of the receptor is not required for cell growth, demonstrating that Stat6 tyrosine phosphorylation does not contribute to mitogenesis.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular Humans Interleukin-3/pharmacology Interleukin-4/pharmacology Mice Mitosis/drug effects Molecular Sequence Data Phosphorylation STAT6 Transcription Factor Sequence Analysis Trans-Activators/genetics,metabolism Transcription Factors/genetics,isolation & purification,metabolism
Chemicals
Interleukin-3 STAT6 Transcription Factor STAT6 protein, human Stat6 protein, mouse Trans-Activators Transcription Factors Interleukin-4
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Quelle F W
Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
Shimoda K
Thierfelder W
Fischer C
Kim A
Ruben S M
Cleveland J L
Pierce J H
Keegan A D
Nelms K
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.6.3336
Published
1995-06-00
Pages
3336-43
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230567
Subset
IM
Grants
NHLBI NIH HHS · P01 HL53749 · United States
NCI NIH HHS · P30 CA21765 · United States
NIDDK NIH HHS · R01 DK44158 · United States
Databases
GENBANK
L47650
External Links
PubMed source DOI: 10.1128/MCB.15.6.3336 PMC Full Text View journal details
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