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PMID: 7751276 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of Lrp-regulated genes by inverse PCR and sequencing: regulation of two mal operons of Escherichia coli by leucine-responsive regulatory protein.

Journal of bacteriology ·Vol. 177 ·No. 10 ·1995-05-00 ·Pages 2679-83

Tchetina E, Newman EB

Abstract

We have used the technique of inverse PCR to identify Escherichia coli chromosomal genes carrying Lrp-regulated inserts. This technique revealed that malT, malEFG, and malB-lamB-malK are all activated two- to fivefold by Lrp and confirmed that Lrp regulates expression of the leuDBCA and livHJKG operons. lacZ transcription is also increased in the presence of Lrp. However, the growth rate of the Lrp mutant on maltose and lactose is not decreased by Lrp deficiency.

MeSH Terms

Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Chromosome Mapping Chromosomes, Bacterial/genetics Cloning, Molecular Escherichia coli/drug effects,genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial Genes, Bacterial/genetics Molecular Sequence Data Mutagenesis, Insertional Open Reading Frames/genetics Oxidation-Reduction Oxidative Stress/genetics Paraquat/pharmacology Promoter Regions, Genetic/genetics RNA, Messenger/genetics Recombinant Fusion Proteins/biosynthesis Sequence Analysis, DNA Trans-Activators Transcription Factors/genetics

Chemicals

Bacterial Proteins Escherichia coli Proteins RNA, Messenger Recombinant Fusion Proteins Trans-Activators Transcription Factors SoxR protein, Bacteria SoxS protein, E coli Paraquat

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Tchetina E

Biology Department, Concordia University, Montreal, Quebec, Canada.

Newman E B

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23 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.177.10.2679-2683.1995

Published

1995-05-00

Pages

2679-83

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC176937

Subset

External Links

PubMed source DOI: 10.1128/jb.177.10.2679-2683.1995 PMC Full Text View journal details

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Identification of Lrp-regulated genes by inverse PCR and sequencing: regulation of two mal operons of Escherichia coli by leucine-responsive regulatory protein.

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