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PMID: 7739888 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Ciliate telomerase RNA structural features.

Nucleic acids research ·Vol. 23 ·No. 7 ·1995-04-11 ·Pages 1091-7

McCormick-Graham M, Romero DP

Abstract

Telomerase RNA is an integral part of telomerase, the ribonucleoprotein enzyme that catalyzes the synthesis of telomeric DNA. The RNA moiety contains a templating domain that directs the synthesis of a species-specific telomeric repeat and may also be important for enzyme structure and/or catalysis. Phylogenetic comparisons of telomerase RNA sequences from various Tetrahymena spp. and hypotrich ciliates have revealed two conserved secondary structure models that share many features. We have cloned and sequenced the telomerase RNA genes from an additional six Tetrahymena spp. (T. vorax, T. borealis, T. australis, T. silvana, T. capricornis and T. paravorax). Inclusion of these sequences, most notably that from T. paravorax, in a phylogenetic comparative analysis allowed us to more narrowly define structural elements that may be necessary for a minimal telomerase RNA. A primary sequence element, positioned 5' of the template and conserved between all previously known ciliate telomerase RNAs, has been reduced from 5'-(C)UGUCA-3' to the 4 nt sequence 5'-GUCA-3'. Conserved secondary structural features and the impact they have on the general organization of ciliate telomerase RNAs is discussed.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA Nucleotidylexotransferase/chemistry,genetics Genes, Protozoan Models, Molecular Molecular Sequence Data Molecular Structure Nucleic Acid Conformation Polymerase Chain Reaction RNA, Protozoan/chemistry,genetics Sequence Homology, Nucleic Acid Species Specificity Tetrahymena/chemistry,enzymology,genetics
Chemicals
RNA, Protozoan DNA Nucleotidylexotransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
McCormick-Graham M
Department of Pharmacology, School of Medicine, University of Minnesota, Minneapolis 55455, USA.
Romero D P
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/23.7.1091
Published
1995-04-11
Pages
1091-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306816
Subset
IM
Grants
NIGMS NIH HHS · GM 50861 · United States
Databases
GENBANK
U22349, U22350, U22351, U22352, U22353, U22354
External Links
PubMed source DOI: 10.1093/nar/23.7.1091 PMC Full Text View journal details
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