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PMID: 7715742 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Okadaic acid modulates exocytotic and transporter-dependent release of dopamine in bovine retina in vitro.

Naunyn-Schmiedeberg's archives of pharmacology ·Vol. 351 ·No. 1 ·1995-01-00 ·Pages 53-9

Bugnon O, Ofori S, Schorderet M

Abstract

Bovine retinas were isolated for the study of the modulation of exocytotic and transporter-dependent release of dopamine (DA) in vitro. Endogenous DA was measured in the medium using HPLC with electrochemical detection under successive incubations with transfers in fresh medium every 30 min. As expected, potassium caused a calcium-dependent exocytotic liberation of DA. Amphetamine or tyramine induced a calcium-independent release by reversing DA transport across the plasma membrane. Okadaic acid, a specific inhibitor of phosphatases 1 and 2A, induced a slight but significant DA release in the absence of calcium. Furthermore, the toxin increased potassium-, amphetamine- or tyramine-induced DA release independently of extracellular calcium. In addition, okadaic acid completely annulled the ability of a calcium-free extracellular environment to inhibit the potassium-induced DA release. Finally, the toxin prevented the time-dependent decline in the efficacies of amphetamine or tyramine to release DA. In agreement with proposed schemes described for rat striatum, the results of the present study confirmed the existence of distinct release modes of DA in bovine retina. The results obtained with okadaic acid suggest that phosphatase 1 and/or phosphatase 2A constitute part of a direct or indirect mechanism to inhibit both exocytotic and transporter-dependent DA release.

MeSH Terms
Animals Calcium/physiology Carrier Proteins/drug effects Cattle Chromatography, High Pressure Liquid Culture Techniques Dextroamphetamine/pharmacology Dopamine/metabolism Ethers, Cyclic/pharmacology Exocytosis/drug effects Okadaic Acid Phosphoprotein Phosphatases/antagonists & inhibitors Potassium/pharmacology Protein Phosphatase 1 Protein Phosphatase 2 Retina/drug effects,physiology Tyramine/pharmacology
Chemicals
Carrier Proteins Ethers, Cyclic Okadaic Acid Phosphoprotein Phosphatases Protein Phosphatase 1 Protein Phosphatase 2 Potassium Calcium Dextroamphetamine Dopamine Tyramine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bugnon O
Département de Pharmacologie, Centre Médical Universitaire, Genève, Switzerland.
Ofori S
Schorderet M
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Article Info
Journal
Naunyn-Schmiedeberg's archives of pharmacology
Abbr.
Naunyn Schmiedebergs Arch Pharmacol
ISSN
0028-1298
DOI
10.1007/BF00169064
Published
1995-01-00
Pages
53-9
Language
English
Region
Germany
NLM ID
0326264
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00169064 View journal details
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