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PMID: 1722321 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cloning of the cocaine-sensitive bovine dopamine transporter.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 24 ·1991-12-15 ·Pages 11168-71

Usdin TB, Mezey E, Chen C, Brownstein MJ, Hoffman BJ

Abstract

A cDNA encoding the dopamine transporter from bovine brain substantia nigra was identified on the basis of its structural homology to other, recently cloned, neurotransmitter transporters. The sequence of the 693-amino acid protein is quite similar to those of the rat gamma-aminobutyric acid, human norepinephrine, and rat serotonin transporters. Dopamine transporter mRNA was detected by in situ hybridization in the substantia nigra but not in the locus coeruleus, raphe, caudate, or other brain areas. [3H]Dopamine accumulation in tissue culture cells transfected with the cDNA was inhibited by amphetamine, cocaine, and specific inhibitors of dopamine transport, including GBR12909.

MeSH Terms
Amino Acid Sequence Animals Antisense Elements (Genetics) Base Sequence Carrier Proteins/drug effects,genetics,metabolism Cattle Cell Line Cloning, Molecular/methods Cocaine/pharmacology Dopamine/metabolism Dopamine Antagonists Dopamine Plasma Membrane Transport Proteins Gene Library Kinetics Membrane Glycoproteins Membrane Transport Proteins Molecular Sequence Data Nerve Tissue Proteins Nucleic Acid Hybridization Piperazines/pharmacology RNA/genetics,isolation & purification RNA Probes Sequence Homology, Nucleic Acid Substantia Nigra/drug effects,physiology Transfection Tyrosine 3-Monooxygenase/genetics
Chemicals
Antisense Elements (Genetics) Carrier Proteins Dopamine Antagonists Dopamine Plasma Membrane Transport Proteins Membrane Glycoproteins Membrane Transport Proteins Nerve Tissue Proteins Piperazines RNA Probes RNA vanoxerine Tyrosine 3-Monooxygenase Cocaine Dopamine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Usdin T B
Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, MD 20892.
Mezey E
Chen C
Brownstein M J
Hoffman B J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.24.11168
Published
1991-12-15
Pages
11168-71
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53095
Subset
IM
Databases
GENBANK
M59745, M59746, M59747, M59748, M80234, M80723, M80724, M81664, M81665, M81666
External Links
PubMed source DOI: 10.1073/pnas.88.24.11168 PMC Full Text View journal details
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