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PMID: 7693654 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcription from two promoters and autoregulation contribute to the control of expression of the Salmonella typhimurium flagellar regulatory gene flgM.

Journal of bacteriology ·Vol. 175 ·No. 21 ·1993-11-00 ·Pages 7006-15

Gillen KL, Hughes KT

Abstract

The flgM gene product has been shown to be a negative regulator of flagellin transcription in Salmonella typhimurium (K. L. Gillen and K. T. Hughes, J. Bacteriol. 173:2301-2310, 6453-6459, 1991; K. Ohnishi, K. Kutsukake, H. Suzuki, and T. Iino, Mol. Microbiol. 6:3149-3157, 1992). Mud-lac fusions to the flgM gene were isolated and used to characterize the regulation of flgM gene expression. Transcription of the flgM gene was decreased more than 30-fold in strains with the flagellar master regulatory genes, flhC and flhD, deleted. A class 2 flagellar defect caused a slight increase of flgM gene transcription unless a wild-type copy of the flgM gene was present, in which case transcription was decreased threefold. A deletion in the gene for the alternative sigma factor sigma 28 (FliA) caused a fourfold decrease in flgM expression. Insertional inactivation of a gene upstream of the flgM gene (flgA) in a fliA mutant strain caused transcription of the flgM gene to be decreased to a basal level. Northern (RNA) blot analysis confirmed the presence of two transcripts through the flgM gene, one which initiates upstream of the flgM gene and a second which initiates upstream of the flgA gene.

Related Genes
flgA flgB flgM flhC flhD fliA
MeSH Terms
Alleles Base Sequence Chromosome Mapping Chromosomes, Bacterial DNA Primers DNA Transposable Elements Flagellin/biosynthesis,genetics Gene Expression Regulation, Bacterial Genes, Regulator Homeostasis Molecular Sequence Data Multigene Family Mutagenesis, Insertional Promoter Regions, Genetic Protein Biosynthesis RNA, Bacterial/biosynthesis,isolation & purification RNA, Messenger/biosynthesis,isolation & purification Salmonella typhimurium/genetics,metabolism Transcription, Genetic
Chemicals
DNA Primers DNA Transposable Elements RNA, Bacterial RNA, Messenger Flagellin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gillen K L
Department of Microbiology, University of Washington, Seattle 98195.
Hughes K T
References (29)
29 references, click to expand
  1. Requirement of adenosine 3', 5'-cyclic phosphate for flagella formation in Escherichia coli and Salmonella typhimurium.
    J Bacteriol. 1970 Aug;103(2):513-6 PMID: 4317316
  2. Genetics and biogenesis of bacterial flagella.
    Annu Rev Genet. 1992;26:131-58 PMID: 1482109
  3. Absence of messenger ribonucleic acid specific for flagellin in non-flagellate mutants of Salmonella.
    J Mol Biol. 1975 Jul 15;95(4):549-56 PMID: 1097717
  4. The role of cAMP in flagellation of Salmonella typhimurium.
    Mol Gen Genet. 1976 Dec 31;142(4):289-98 PMID: 178991
  5. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
    Biochemistry. 1977 Oct 18;16(21):4743-51 PMID: 911786
  6. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  7. Incomplete flagellar structures in Escherichia coli mutants.
    J Bacteriol. 1981 Feb;145(2):1036-41 PMID: 7007337
  8. Role of the flaR gene in flagellar hook formation in Salmonella spp.
    J Bacteriol. 1981 Dec;148(3):973-9 PMID: 7309683
  9. Fusions of flagellar operons to lactose genes on a mu lac bacteriophage.
    J Bacteriol. 1982 Apr;150(1):16-26 PMID: 7037746
  10. DNA sequence organization of IS10-right of Tn10 and comparison with IS10-left.
    Proc Natl Acad Sci U S A. 1982 Apr;79(8):2608-12 PMID: 6283536
  11. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  12. Linkage map of Salmonella typhimurium, Edition VI.
    Microbiol Rev. 1983 Sep;47(3):410-53 PMID: 6314110
  13. Genomic sequencing.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 PMID: 6326095
  14. Directed formation of deletions and duplications using Mud(Ap, lac).
    Genetics. 1985 Feb;109(2):263-82 PMID: 3156064
  15. New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition.
    Gene. 1984 Dec;32(3):369-79 PMID: 6099322
  16. Locations of hook-associated proteins in flagellar structures of Salmonella typhimurium.
    J Bacteriol. 1985 Apr;162(1):183-9 PMID: 3884587
  17. Transcriptional control of flagellar genes in Escherichia coli K-12.
    J Bacteriol. 1986 Dec;168(3):1315-8 PMID: 3536871
  18. Formation of flagella lacking outer rings by flaM, flaU, and flaY mutants of Escherichia coli.
    J Bacteriol. 1987 Apr;169(4):1485-8 PMID: 3549689
  19. Identification of flagellar hook and basal body gene products (FlaFV, FlaFVI, FlaFVII and FlaFVIII) in Salmonella typhimurium.
    J Bacteriol. 1987 Aug;169(8):3617-24 PMID: 3301807
  20. Packaging specific segments of the Salmonella chromosome with locked-in Mud-P22 prophages.
    Genetics. 1988 Apr;118(4):581-92 PMID: 2835289
  21. Transitory cis complementation: a method for providing transposition functions to defective transposons.
    Genetics. 1988 May;119(1):9-12 PMID: 2840333
  22. Transcriptional analysis of the flagellar regulon of Salmonella typhimurium.
    J Bacteriol. 1990 Feb;172(2):741-7 PMID: 2404955
  23. Gene fliA encodes an alternative sigma factor specific for flagellar operons in Salmonella typhimurium.
    Mol Gen Genet. 1990 Apr;221(2):139-47 PMID: 2196428
  24. Transposon-facilitated DNA sequencing.
    Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1247-50 PMID: 1847513
  25. Negative regulatory loci coupling flagellin synthesis to flagellar assembly in Salmonella typhimurium.
    J Bacteriol. 1991 Apr;173(7):2301-10 PMID: 1848842
  26. Molecular characterization of flgM, a gene encoding a negative regulator of flagellin synthesis in Salmonella typhimurium.
    J Bacteriol. 1991 Oct;173(20):6453-9 PMID: 1655712
  27. Subdivision of flagellar region III of the Escherichia coli and Salmonella typhimurium chromosomes and identification of two additional flagellar genes.
    J Gen Microbiol. 1992 Jun;138(6):1051-65 PMID: 1527488
  28. A novel transcriptional regulation mechanism in the flagellar regulon of Salmonella typhimurium: an antisigma factor inhibits the activity of the flagellum-specific sigma factor, sigma F.
    Mol Microbiol. 1992 Nov;6(21):3149-57 PMID: 1453955
  29. Characterization of Escherichia coli flagellar mutants that are insensitive to catabolite repression.
    J Bacteriol. 1974 Dec;120(3):1196-203 PMID: 4373438
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.21.7006-7015.1993
Published
1993-11-00
Pages
7006-15
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206828
Subset
IM
Grants
NIGMS NIH HHS · GM43149 · United States
NIGMS NIH HHS · T32 GM07270 · United States
External Links
PubMed source DOI: 10.1128/jb.175.21.7006-7015.1993 PMC Full Text View journal details
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