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PMID: 7690545 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ribosome-binding protein p34 is a member of the leucine-rich-repeat-protein superfamily.

The Biochemical journal ·Vol. 294 ( Pt 2) ·1993-09-01 ·Pages 465-72

Ohsumi T, Ichimura T, Sugano H, Omata S, Isobe T, Kuwano R

Abstract

Protein p34 is a non-glycosylated membrane protein characteristic of rough microsomes and is believed to play a role in the ribosome-membrane association. In the present study we isolated cDNA encoding p34 from a rat liver cDNA library and determined its complete amino acid sequence. p34 mRNA is 3.2 kb long and encodes a polypeptide of 307 amino acids with a molecular mass of about 34.9 kDa. Primary sequence analysis, coupled with biochemical studies on the topology, suggested that p34 is a type II signal-anchor protein; it is composed of a large cytoplasmic domain, a membrane-spanning segment and a 38-amino-acid-long luminally disposed C-terminus. The cytoplasmic domain of p34 has several noteworthy structural features, including a region of 4.5 tandem repeats of 23-24 amino acids. The repeated motif shows structural similarity to the leucine-rich repeat which is found in a variety of proteins widely distributed among eukaryotic cells and which potentially functions in mediating protein-protein interactions. The cytoplasmic domain also contains a characteristic hydrophilic region with abundant charged amino acids. These structural regions may be important for the observed ribosome-binding activity of the p34 protein.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites Carrier Proteins/analysis,chemistry,genetics Consensus Sequence DNA/chemistry,isolation & purification Leucine/analysis Membrane Proteins/analysis,chemistry,genetics Molecular Sequence Data Molecular Weight RNA/metabolism Rats Repetitive Sequences, Nucleic Acid Ribosomes/metabolism Sequence Homology
Chemicals
Carrier Proteins Membrane Proteins ribosome-binding protein p34, rat RNA DNA Leucine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ohsumi T
Department of Biosystem Science, Graduate School of Science and Technology, Niigata University, Japan.
Ichimura T
Sugano H
Omata S
Isobe T
Kuwano R
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2940465
Published
1993-09-01
Pages
465-72
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1134477
Subset
IM
Databases
GENBANK
D13623, D13666, D17642, D17799, D17800, D17801, D17802, M99422, X71481, X71774
External Links
PubMed source DOI: 10.1042/bj2940465 PMC Full Text View journal details
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