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PMID: 2189727 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Drosophila chaoptin, a member of the leucine-rich repeat family, is a photoreceptor cell-specific adhesion molecule.

The EMBO journal ·Vol. 9 ·No. 6 ·1990-06-00 ·Pages 1969-77

Krantz DE, Zipursky SL

Abstract

Drosophila chaoptin, required for photoreceptor cell morphogenesis, is a member of the leucine-rich repeat family of proteins. On the basis of biochemical and genetic analyses we previously proposed that chaoptin might function as a cell adhesion molecule. To test this hypothesis, chaoptin cDNA driven by the hsp 70 promoter was transfected into non-self-adherent Drosophila Schneider line 2 (S2) cells. Following heat shock induction of chaoptin expression, the transfected S2 cells formed multicellular aggregates. Mixing experiments of chaoptin expressing and non-expressing cells suggest that chaoptin expressing cells adhere homotypically. Previously it was shown that chaoptin is exclusively localized to photoreceptor cells. Thus, chaoptin is a cell-type-specific adhesion molecule. Biochemical analyses presented in this paper demonstrate that chaoptin is linked to the extracellular surface of the plasma membrane by covalent attachment to glycosyl-phosphatidylinositol. We propose that chaoptin and several other members of the leucine-rich repeat family of proteins define a new class of cell adhesion molecules.

MeSH Terms
Animals Cell Adhesion Cell Aggregation/drug effects Cell Membrane/metabolism Cells, Cultured DNA/genetics Drosophila/genetics,ultrastructure Drosophila Proteins Glycolipids/physiology Leucine/genetics Membrane Glycoproteins/genetics,immunology Multigene Family Photoreceptor Cells/metabolism Sensitivity and Specificity Time Factors Type C Phospholipases/pharmacology
Chemicals
Drosophila Proteins Glycolipids Membrane Glycoproteins chp protein, Drosophila DNA Type C Phospholipases Leucine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Krantz D E
Department of Biological Chemistry, UCLA School of Medicine.
Zipursky S L
References (61)
61 references, click to expand
  1. N-cadherin and integrins: two receptor systems that mediate neuronal process outgrowth on astrocyte surfaces.
    Neuron. 1988 Mar;1(1):33-43 PMID: 2856086
  2. Drosophila neuroglian: a member of the immunoglobulin superfamily with extensive homology to the vertebrate neural adhesion molecule L1.
    Cell. 1989 Nov 3;59(3):447-60 PMID: 2805067
  3. Adhesion molecules and the hierarchy of neural development.
    Neuron. 1988 Mar;1(1):3-13 PMID: 3078409
  4. Cell lines derived from late embryonic stages of Drosophila melanogaster.
    J Embryol Exp Morphol. 1972 Apr;27(2):353-65 PMID: 4625067
  5. Adhesion among neural cells of the chick embryo. I. An immunological assay for molecules involved in cell-cell binding.
    J Biol Chem. 1977 Oct 10;252(19):6835-40 PMID: 893445
  6. DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1373-6 PMID: 286319
  7. Recognition of cell types by axonal growth cones in vitro.
    Nature. 1980 Nov 13;288(5787):162-4 PMID: 7432513
  8. Monoclonal antibodies against the Drosophila nervous system.
    Proc Natl Acad Sci U S A. 1982 Dec;79(24):7929-33 PMID: 6818557
  9. Neuronal development in the Drosophila retina: monoclonal antibodies as molecular probes.
    Cell. 1984 Jan;36(1):15-26 PMID: 6420071
  10. From monoclonal antibody to gene for a neuron-specific glycoprotein in Drosophila.
    Proc Natl Acad Sci U S A. 1985 Mar;82(6):1855-9 PMID: 3920657
  11. Periodicity of leucine and tandem repetition of a 24-amino acid segment in the primary structure of leucine-rich alpha 2-glycoprotein of human serum.
    Proc Natl Acad Sci U S A. 1985 Apr;82(7):1906-10 PMID: 3856868
  12. Isolation and structure of a rhodopsin gene from D. melanogaster.
    Cell. 1985 Apr;40(4):851-8 PMID: 2580638
  13. Folding patterns of porin and bacteriorhodopsin.
    EMBO J. 1985 Jun;4(6):1593-7 PMID: 2992935
  14. Structure and function of platelet membrane glycoproteins Ib and V. Effects of leukocyte elastase and other proteases on platelets response to von Willebrand factor and thrombin.
    Eur J Biochem. 1985 Nov 15;153(1):1-11 PMID: 2933256
  15. Identification and purification of membrane and soluble forms of the major surface protein of Leishmania promastigotes.
    J Biol Chem. 1985 Dec 15;260(29):15504-9 PMID: 4066680
  16. DNA sequence and characterization of the S. cerevisiae gene encoding adenylate cyclase.
    Cell. 1985 Dec;43(2 Pt 1):493-505 PMID: 2934138
  17. The von Willebrand factor-binding domain of platelet membrane glycoprotein Ib. Characterization by monoclonal antibodies and partial amino acid sequence analysis of proteolytic fragments.
    J Biol Chem. 1986 Sep 25;261(27):12579-85 PMID: 2943738
  18. Transformation of cultured Drosophila melanogaster cells with a dominant selectable marker.
    Mol Cell Biol. 1985 Aug;5(8):1833-8 PMID: 3018529
  19. An E. coli beta-galactosidase cassette suitable for study of eukaryotic expression.
    Nucleic Acids Res. 1986 Sep 11;14(17):7130 PMID: 3093988
  20. Primary structure of an extracellular matrix proteoglycan core protein deduced from cloned cDNA.
    Proc Natl Acad Sci U S A. 1986 Oct;83(20):7683-7 PMID: 3484330
  21. Phosphatidylinositol is involved in the membrane attachment of NCAM-120, the smallest component of the neural cell adhesion molecule.
    EMBO J. 1986 Oct;5(10):2489-94 PMID: 3780668
  22. cDNA clones of the neural cell adhesion molecule (N-CAM) lacking a membrane-spanning region consistent with evidence for membrane attachment via a phosphatidylinositol intermediate.
    Proc Natl Acad Sci U S A. 1986 Dec;83(24):9822-6 PMID: 3467341
  23. Integrins: a family of cell surface receptors.
    Cell. 1987 Feb 27;48(4):549-54 PMID: 3028640
  24. Expression of fasciclin I and II glycoproteins on subsets of axon pathways during neuronal development in the grasshopper.
    Cell. 1987 Mar 13;48(5):745-55 PMID: 3545496
  25. Functional correlation between cell adhesive properties and some cell surface proteins.
    J Cell Biol. 1977 Nov;75(2 Pt 1):464-74 PMID: 264120
  26. Redesigning the body plan of Drosophila by ectopic expression of the homoeotic gene Antennapedia.
    Nature. 1987 Feb 26-Mar 4;325(6107):816-8 PMID: 3821869
  27. Release of the 120 kDa component of the mouse neural cell adhesion molecule N-CAM from cell surfaces by phosphatidylinositol-specific phospholipase C.
    Neurosci Lett. 1986 Dec 23;72(3):341-6 PMID: 3822237
  28. Neural cell adhesion molecule: structure, immunoglobulin-like domains, cell surface modulation, and alternative RNA splicing.
    Science. 1987 May 15;236(4803):799-806 PMID: 3576199
  29. Amino acid sequence of the von Willebrand factor-binding domain of platelet membrane glycoprotein Ib.
    Proc Natl Acad Sci U S A. 1987 Aug;84(16):5610-4 PMID: 3497398
  30. Cloning of the alpha chain of human platelet glycoprotein Ib: a transmembrane protein with homology to leucine-rich alpha 2-glycoprotein.
    Proc Natl Acad Sci U S A. 1987 Aug;84(16):5615-9 PMID: 3303030
  31. Transformation of cell adhesion properties by exogenously introduced E-cadherin cDNA.
    Nature. 1987 Sep 24-30;329(6137):341-3 PMID: 3498123
  32. Biochemistry of the glycosyl-phosphatidylinositol membrane protein anchors.
    Biochem J. 1987 May 15;244(1):1-13 PMID: 2959270
  33. Scrapie prion protein contains a phosphatidylinositol glycolipid.
    Cell. 1987 Oct 23;51(2):229-40 PMID: 2444340
  34. Cellular expression of liver and neural cell adhesion molecules after transfection with their cDNAs results in specific cell-cell binding.
    Proc Natl Acad Sci U S A. 1987 Dec;84(23):8502-6 PMID: 3317412
  35. Cell adhesion in morphogenesis.
    Annu Rev Cell Biol. 1987;3:319-45 PMID: 3318879
  36. Structural and functional roles of glycosyl-phosphatidylinositol in membranes.
    Science. 1988 Jan 15;239(4837):268-75 PMID: 3276003
  37. The Toll gene of Drosophila, required for dorsal-ventral embryonic polarity, appears to encode a transmembrane protein.
    Cell. 1988 Jan 29;52(2):269-79 PMID: 2449285
  38. Analysis of mutants in chaoptin, a photoreceptor cell-specific glycoprotein in Drosophila, reveals its role in cellular morphogenesis.
    Cell. 1988 Jan 29;52(2):281-90 PMID: 2449286
  39. Chaoptin, a cell surface glycoprotein required for Drosophila photoreceptor cell morphogenesis, contains a repeat motif found in yeast and human.
    Cell. 1988 Jan 29;52(2):291-301 PMID: 3124963
  40. The alpha and beta chains of human platelet glycoprotein Ib are both transmembrane proteins containing a leucine-rich amino acid sequence.
    Proc Natl Acad Sci U S A. 1988 Apr;85(7):2135-9 PMID: 3353370
  41. A phosphatidylinositol-linked peanut agglutinin-binding glycoprotein in central nervous system myelin and on oligodendrocytes.
    J Cell Biol. 1988 Apr;106(4):1273-9 PMID: 3283151
  42. The oligodendrocyte-myelin glycoprotein belongs to a distinct family of proteins and contains the HNK-1 carbohydrate.
    J Cell Biol. 1990 Feb;110(2):471-9 PMID: 1688857
  43. Mutations of the adenylyl cyclase gene that block RAS function in Saccharomyces cerevisiae.
    Science. 1990 Jan 26;247(4941):464-7 PMID: 2405488
  44. Genetic analysis of a Drosophila neural cell adhesion molecule: interaction of fasciclin I and Abelson tyrosine kinase mutations.
    Cell. 1990 Feb 23;60(4):565-75 PMID: 2406026
  45. Characterization and cloning of fasciclin III: a glycoprotein expressed on a subset of neurons and axon pathways in Drosophila.
    Cell. 1987 Mar 27;48(6):975-88 PMID: 3548998
  46. Neurite outgrowth on muscle cell surfaces involves extracellular matrix receptors as well as Ca2+-dependent and -independent cell adhesion molecules.
    Proc Natl Acad Sci U S A. 1987 Apr;84(8):2555-9 PMID: 3031688
  47. The immunoglobulin superfamily--domains for cell surface recognition.
    Annu Rev Immunol. 1988;6:381-405 PMID: 3289571
  48. Characterization and cloning of fasciclin I and fasciclin II glycoproteins in the grasshopper.
    Proc Natl Acad Sci U S A. 1988 Jul;85(14):5291-5 PMID: 2839842
  49. Cell adhesion molecules in vertebrate neural development.
    Physiol Rev. 1988 Jul;68(3):819-57 PMID: 3293093
  50. Zygotic expression and activity of the Drosophila Toll gene, a gene required maternally for embryonic dorsal-ventral pattern formation.
    Genetics. 1988 May;119(1):123-33 PMID: 2456252
  51. Expressed recombinant cadherins mediate cell sorting in model systems.
    Cell. 1988 Sep 23;54(7):993-1001 PMID: 3416359
  52. The cadherins: cell-cell adhesion molecules controlling animal morphogenesis.
    Development. 1988 Apr;102(4):639-55 PMID: 3048970
  53. Cell-surface anchoring of proteins via glycosyl-phosphatidylinositol structures.
    Annu Rev Biochem. 1988;57:285-320 PMID: 3052274
  54. Prion protein biosynthesis in scrapie-infected and uninfected neuroblastoma cells.
    J Virol. 1989 Jan;63(1):175-81 PMID: 2562814
  55. Amino acid sequence of the ribonuclease inhibitor from porcine liver reveals the presence of leucine-rich repeats.
    Biochemistry. 1988 Nov 15;27(23):8537-44 PMID: 3219361
  56. Primary structure of human placental ribonuclease inhibitor.
    Biochemistry. 1988 Nov 15;27(23):8545-53 PMID: 3219362
  57. Deduced protein sequence of bone small proteoglycan I (biglycan) shows homology with proteoglycan II (decorin) and several nonconnective tissue proteins in a variety of species.
    J Biol Chem. 1989 Mar 15;264(8):4571-6 PMID: 2647739
  58. Analysis of the gene encoding the largest subunit of RNA polymerase II in Drosophila.
    Mol Gen Genet. 1989 Jan;215(2):266-75 PMID: 2496296
  59. Lutropin-choriogonadotropin receptor: an unusual member of the G protein-coupled receptor family.
    Science. 1989 Aug 4;245(4917):494-9 PMID: 2502842
  60. Fasciclin III: a novel homophilic adhesion molecule in Drosophila.
    Cell. 1989 Oct 20;59(2):313-23 PMID: 2509076
  61. Unwrapping the genes of myelin.
    Neuron. 1988 Sep;1(7):535-43 PMID: 2483101
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-06-00
Pages
1969-77
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551906
Subset
IM
Grants
NIGMS NIH HHS · GM37365 · United States
External Links
PubMed source PMC Full Text View journal details
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