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PMID: 7669572 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of N-cadherin and alpha-catenin in astrocytomas and glioblastomas.

British journal of cancer ·Vol. 72 ·No. 3 ·1995-09-00 ·Pages 627-33

Shinoura N, Paradies NE, Warnick RE, Chen H, Larson JJ, Tew JJ, Simon M, Lynch RA, Kanai Y, Hirohashi S

Abstract

We examined levels of mRNA and protein for N-cadherin, the predominant cadherin in neural tissues, and mRNA levels for the cadherin-associated protein, alpha-catenin, in a series of gliomas and in glioblastoma cell lines. mRNA levels for N-cadherin and alpha-catenin were significantly higher in glioblastomas than in low-grade astrocytomas or normal brain, while the levels of intact N-cadherin protein were similar in glioblastomas, low-grade astrocytomas and brain. In addition, there was no consistent relationship between invasiveness and expression of N-cadherin and alpha-catenin in highly invasive vs minimally invasive tumours within the same histopathological grade. To assess further the relationship between cadherin expression and neural tumour invasion, we measured N-cadherin expression, calcium-dependent cell adhesion and motility of several glioblastoma cell lines. While all N-cadherin-expressing lines were adhesive, no correlation was seen between the level of N-cadherin expression and cell motility. Together, these findings imply that, in contrast to the role played by E-cadherin in carcinomas, N-cadherin does not restrict the invasion of glioblastomas.

MeSH Terms
Astrocytoma/genetics,pathology Base Sequence Brain/metabolism Brain Neoplasms/genetics,pathology Cadherins/genetics Cell Adhesion/physiology Cell Aggregation/physiology Cell Movement/physiology Cytoskeletal Proteins/genetics Gene Expression Glioblastoma/genetics,pathology Humans Molecular Sequence Data Neoplasm Invasiveness Neoplasm Proteins/genetics RNA, Messenger/metabolism Reference Values Tumor Cells, Cultured alpha Catenin
Chemicals
CTNNA1 protein, human Cadherins Cytoskeletal Proteins Neoplasm Proteins RNA, Messenger alpha Catenin
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Shinoura N
Department of Molecular Genetics, University of Cincinnati, College of Medicine, Ohio 45267, USA.
Paradies N E
Warnick R E
Chen H
Larson J J
Tew J J
Simon M
Lynch R A
Kanai Y
Hirohashi S
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Article Info
Journal
British journal of cancer
Abbr.
Br J Cancer
ISSN
0007-0920
DOI
10.1038/bjc.1995.384
Published
1995-09-00
Pages
627-33
Language
English
Region
England
NLM ID
0370635
PMCID
PMC2033884
Subset
IM
Grants
NCI NIH HHS · CA59268 · United States
NINDS NIH HHS · P20-NS31145 · United States
External Links
PubMed source DOI: 10.1038/bjc.1995.384 PMC Full Text View journal details
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