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PMID: 7647301 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Nature and regulation of pistil-expressed genes in tomato.

Plant molecular biology ·Vol. 28 ·No. 4 ·1995-07-00 ·Pages 691-711

Milligan SB, Gasser CS

Abstract

The specialized reproductive functions of angiosperm pistils are dependent in part upon the regulated activation of numerous genes expressed predominantly in this organ system. To better understand the nature of these pistil-predominant gene products we have analyzed seven cDNA clones isolated from tomato pistils through differential hybridization screening. Six of the seven cDNAs represent sequences previously undescribed in tomato, each having a unique pistil- and/or floral-predominant expression pattern. The putative protein products encoded by six of the cDNAs have been identified by their similarity to sequences in the database of previously sequenced genes, with a seventh sequence having no significant similarity with any previously reported sequence. Three of the putative proteins appear to be targeted to the endomembrane system and include an endo-beta-1,4-glucanase which is expressed exclusively in pistils at early stages of development, and proteins similar in sequence to gamma-thionin and miraculin which are expressed in immature pistils and stamens, and in either sepals or petals, respectively. Two other clones, similar in sequence to each other, were expressed primarily in immature pistils and stamens and encode distinct proteins with similarity to leucine aminopeptidases. An additional clone, which encodes a protein similar in sequence to the enzyme hyoscyamine 6-beta-hydroxylase and to other members of the family of Fe2+/ascorbate-dependent oxidases, was expressed at high levels in pistils, stamens and sepals, and at detectable levels in some vegetative organs. Together, these observations provide new insight into the nature and possible functional roles of genes expressed during reproductive development.

MeSH Terms
Amino Acid Sequence Antimicrobial Cationic Peptides Blotting, Northern Cellulase/genetics DNA, Complementary/genetics Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Gene Library Genes, Plant/genetics Glycoproteins/genetics Leucyl Aminopeptidase/genetics Lycopersicon esculentum/genetics,growth & development Molecular Sequence Data Plant Proteins/genetics RNA, Messenger/isolation & purification Reproduction/genetics Sequence Analysis, DNA Sequence Homology, Amino Acid Time Factors Tissue Distribution
Chemicals
Antimicrobial Cationic Peptides DNA, Complementary Glycoproteins Plant Proteins RNA, Messenger miraculin protein, Synsepalum dulcificum FST protein, Nicotiana tabacum Cellulase Leucyl Aminopeptidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Milligan S B
Section of Molecular and Cellular Biology, University of California, Davis 95616, USA.
Gasser C S
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00021194
Published
1995-07-00
Pages
691-711
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
U20590, U20591, U20592, U20593, U20594, U20595, U20596
External Links
PubMed source DOI: 10.1007/BF00021194 View journal details
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