Avocado cellulase: nucleotide sequence of a putative full-length cDNA clone and evidence for a small gene family.

Plant molecular biology ·Vol. 9 ·No. 3 ·1987-05-00 ·Pages 197-203

Tucker ML, Durbin ML, Clegg MT, Lewis LN

Abstract

A cDNA library was prepared from ripe avocado fruit (Persea americana Mill. cv. Hass) and screened for clones hybridizing to a 600 bp cDNA clone (pAV5) coding for avocado fruit cellulase. This screening led to the isolation of a clone (pAV363) containing a 2021 nucleotide transcribed sequence and an approximately 150 nucleotide poly(A) tail. Hybridization of pAV363 to a northern blot shows that the length of the homologous message is approximately 2.2 kb. The nucleotide sequence of this putative full-length mRNA clone contains an open reading frame of 1482 nucleotides which codes for a polypeptide of 54.1 kD. The deduced amino acid composition compares favorably with the amino acid composition of native avocado cellulase determined by amino acid analysis. Southern blot analysis of Hind III and Eco RI endonuclease digested genomic DNA indicates a small family of cellulase genes.

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Tucker M L

Division of Molecular Plant Biology, University of California, Hilgard Hall, 94720, Berkeley, CA, USA.

Durbin M L

Clegg M T

Lewis L N

References (17)

17 references, click to expand

A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA.
Plasmid. 1985 Jan;13(1):31-40 PMID: 3991809
Patterns of amino acids near signal-sequence cleavage sites.
Eur J Biochem. 1983 Jun 1;133(1):17-21 PMID: 6852022
Cellulase gene expression in ripening avocado fruit: The accumulation of cellulase mRNA and protein as demonstrated by cDNA hybridization and immunodetection.
Plant Mol Biol. 1984 Nov;3(6):385-91 PMID: 24310572
Interrelationship of Gene Expression, Polysome Prevalence, and Respiration during Ripening of Ethylene and/or Cyanide-Treated Avocado Fruit.
Plant Physiol. 1984 Feb;74(2):307-15 PMID: 16663414
The DNA sequence of the gene and genetic control sites for the excreted B. subtilis enzyme beta-glucanase.
Nucleic Acids Res. 1984 Jul 11;12(13):5355-67 PMID: 6087283
Extraction of nucleic acids from agarose gels.
Anal Biochem. 1980 Apr;103(2):264-71 PMID: 6155806
Transformation of E. coli using homopolymer-linked plasmid chimeras.
Biochim Biophys Acta. 1981 Sep 28;655(2):243-50 PMID: 7025909
Synthesis and processing of cellulase from ripening avocado fruit.
Plant Physiol. 1986 Jul;81(3):830-5 PMID: 16664911
Assessment of protein sequence identity from amino acid composition data.
J Theor Biol. 1977 Apr 21;65(4):735-42 PMID: 875402
Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics.
Proc Natl Acad Sci U S A. 1984 Dec;81(24):8014-8 PMID: 6096873
A simple and very efficient method for generating cDNA libraries.
Gene. 1983 Nov;25(2-3):263-9 PMID: 6198242
Postharvest Variation in Cellulase, Polygalacturonase, and Pectinmethylesterase in Avocado (Persea americana Mill, cv. Fuerte) Fruits in Relation to Respiration and Ethylene Production.
Plant Physiol. 1979 Aug;64(2):306-8 PMID: 16660954
Nucleotide sequences of two cellulase genes from alkalophilic Bacillus sp. strain N-4 and their strong homology.
J Bacteriol. 1986 Nov;168(2):479-85 PMID: 3782013
Sequence of a cellulase gene of the thermophilic bacterium Clostridium thermocellum.
J Bacteriol. 1985 Apr;162(1):102-5 PMID: 3980433
DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Transformation and preservation of competent bacterial cells by freezing.
Methods Enzymol. 1979;68:326-31 PMID: 396437
Improved yield of full-length phaseolin cDNA clones by controlling premature anticomplementary DNA synthesis.
Plant Mol Biol. 1983 Mar;2(2):75-83 PMID: 24318139

Article Info

Journal

Plant molecular biology

Abbr.

Plant Mol Biol

ISSN

0167-4412

DOI

10.1007/BF00166456

Published

1987-05-00

Pages

197-203

Language

English

Region

Netherlands

NLM ID

9106343

External Links

PubMed source DOI: 10.1007/BF00166456 View journal details

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Avocado cellulase: nucleotide sequence of a putative full-length cDNA clone and evidence for a small gene family.

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