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PMID: 7637037 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Tumor necrosis factor expression during mouse hepatitis virus-induced demyelinating encephalomyelitis.

Journal of virology ·Vol. 69 ·No. 9 ·1995-09-00 ·Pages 5898-903

Stohlman SA, Hinton DR, Cua D, Dimacali E, Sensintaffar J, Hofman FM, Tahara SM, Yao Q

Abstract

Neutralizing anti-tumor necrosis factor alpha (TNF-alpha) antibody treatment of mice infected with the neurotropic JHMV strain of mouse hepatitis virus showed no reduction of either virus-induced encephalomyelitis or central nervous system demyelination. TNF-alpha-positive cells were present in the central nervous system during infection; however, TNF-alpha could not be colocalized with JHMV-infected cells. In vitro, TNF-alpha mRNA rapidly accumulated following JHMV infection; however, no TNF-alpha was secreted because of inhibition of translation. Both live and UV-inactivated virus inhibited TNF-alpha secretion induced by lipopolysaccharide. These data show that TNF-alpha is not secreted from infected cells and indicate that if contributes to either JHMV-induced acute encephalomyelitis nor primary demyelination.

MeSH Terms
Animals Antibodies Blotting, Western Demyelinating Diseases/pathology,physiopathology,virology Encephalomyelitis/pathology,physiopathology,virology Gene Expression Lipopolysaccharides/pharmacology Macrophages/pathology,physiology,virology Mice Mice, Inbred BALB C Mice, Inbred C57BL Murine hepatitis virus/isolation & purification,pathogenicity,radiation effects RNA, Messenger/analysis,biosynthesis Species Specificity Spinal Cord/pathology,physiopathology,virology Tumor Necrosis Factor-alpha/biosynthesis Ultraviolet Rays
Chemicals
Antibodies Lipopolysaccharides RNA, Messenger Tumor Necrosis Factor-alpha
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Stohlman S A
Department of Neurology, University of Southern California School of Medicine, Los Angeles 90033, USA.
Hinton D R
Cua D
Dimacali E
Sensintaffar J
Hofman F M
Tahara S M
Yao Q
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.69.9.5898-5903.1995
Published
1995-09-00
Pages
5898-903
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC189470
Subset
IM
Grants
NIAID NIH HHS · AI7078 · United States
NINDS NIH HHS · NS 30880 · United States
NINDS NIH HHS · NS18146 · United States
External Links
PubMed source DOI: 10.1128/JVI.69.9.5898-5903.1995 PMC Full Text View journal details
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