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PMID: 1548758 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Induction of interleukin-1 and tumor necrosis factor alpha in brain cultures by human immunodeficiency virus type 1.

Journal of virology ·Vol. 66 ·No. 4 ·1992-04-00 ·Pages 2217-25

Merrill JE, Koyanagi Y, Zack J, Thomas L, Martin F, Chen IS

Abstract

Cytokines such as interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF alpha) are produced by leukocytes and play a role in immune responses. They also function in normal brain physiology as well as in pathological conditions within the central nervous system, where they are produced by brain macrophages (microglia) and brain astrocytes. In this study, we document the ability of human immunodeficiency virus type 1 (HIV-1) to induce TNF alpha and IL-1 in primary rat brain cultures. While productive infection did not occur in these cells, it was not required for cytokine induction. Using monocyte/macrophage-tropic (JRFL) and T-cell-tropic (IIIB) strains of HIV-1, we were able to induce cytokines in both microglia and astrocytes. In addition to whole virus, recombinant envelope proteins also induced these cytokines. The induction of IL-1 and TNF alpha could be blocked by a panel of antibodies recognizing epitopes in the gp120 and gp41 areas of the envelope. Soluble recombinant CD4 did not block TNF alpha and IL-1 production. If TNF alpha and IL-1 can be induced in brain tissue by HIV-1, they may contribute to some of the neurologic disorders associated with AIDS.

MeSH Terms
Animals Brain/immunology,microbiology Cells, Cultured HIV-1/immunology Interleukin-1/biosynthesis Kinetics Polymerase Chain Reaction Rats Tumor Necrosis Factor-alpha/biosynthesis Virus Replication
Chemicals
Interleukin-1 Tumor Necrosis Factor-alpha
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Merrill J E
Department of Neurology, Reed Neurological Research Center, UCLA School of Medicine 90024-1678.
Koyanagi Y
Zack J
Thomas L
Martin F
Chen I S
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.4.2217-2225.1992
Published
1992-04-00
Pages
2217-25
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC289014
Subset
IM
Grants
NIAID NIH HHS · AI 30898 · United States
NINDS NIH HHS · NS 25508 · United States
NINDS NIH HHS · R0-1 NS26983 · United States
External Links
PubMed source DOI: 10.1128/JVI.66.4.2217-2225.1992 PMC Full Text View journal details
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