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PMID: 7603434 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A two-element Enhancer-Inhibitor transposon system in Arabidopsis thaliana.

Molecular & general genetics : MGG ·Vol. 247 ·No. 5 ·1995-06-10 ·Pages 555-64

Aarts MG, Corzaan P, Stiekema WJ, Pereira A

Abstract

The Enhancer-Inhibitor (En-I), also known as Suppressor-mutator (Spm-dSpm), transposable element system of maize was modified and introduced into Arabidopsis by Agrobacterium tumefaciens transformation. A stable En/Spm transposase source under control of the CaMV 35S promoter mediated frequent transposition of I/dSpm elements. Transposition occurred continuously throughout plant development over at least seven consecutive plant generations after transformation. New insertions were found at both linked and unlinked positions relative to a transposon donor site. The independent transposition frequency was defined as a transposition parameter, which quantified the rate of unique insertion events and ranged from 7.8% to 29.2% in different populations. An increase as well as a decrease in I/dSpm element copy number was seen at the individual plant level, but not at the population level after several plant generations. The continuous, frequent transposition observed for this transposon system makes it an attractive tool for use in gene tagging in Arabidopsis.

MeSH Terms
Arabidopsis/genetics Chromosome Mapping DNA Transposable Elements/genetics Genes, Plant/genetics Nucleotidyltransferases/genetics Transposases
Chemicals
DNA Transposable Elements Nucleotidyltransferases Transposases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Aarts M G
Department of Molecular Biology, DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), Wageningen, The Netherlands.
Corzaan P
Stiekema W J
Pereira A
References (38)
38 references, click to expand
  1. Molecular analysis of the En/Spm transposable element system of Zea mays.
    EMBO J. 1986 May;5(5):835-41 PMID: 15957213
  2. The maize transposable element system Ac/Ds as a mutagen in Arabidopsis: identification of an albino mutation induced by Ds insertion.
    Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10370-4 PMID: 8234300
  3. Frequent loss of the En transposable element after excision and its relation to chromosome replication in maize (Zea mays L.).
    Genetics. 1994 Feb;136(2):653-71 PMID: 8150289
  4. The tnpA and tnpD gene products of the Spm element are required for transposition in tobacco.
    Plant Cell. 1991 Jan;3(1):73-85 PMID: 1668614
  5. Linked and unlinked transposition of a genetically marked Dissociation element in transgenic tomato.
    Genetics. 1993 Jun;134(2):571-84 PMID: 8100787
  6. Phenotypic and karyotypic status of Beta vulgaris plants regenerated from direct organogenesis in petiole culture.
    Theor Appl Genet. 1989 Apr;77(4):462-8 PMID: 24232710
  7. Phenotypic assay for excision of the maize controlling element Ac in tobacco.
    EMBO J. 1987 Jun;6(6):1547-54 PMID: 16453771
  8. Cloning of the bronze locus in maize by a simple and generalizable procedure using the transposable controlling element Activator (Ac).
    Proc Natl Acad Sci U S A. 1984 Jun;81(12):3825-9 PMID: 16593478
  9. Twin Mutations in Medium Variegated Pericarp Maize.
    Genetics. 1962 Apr;47(4):489-501 PMID: 17248099
  10. Transgenic tomato lines containing Ds elements at defined genomic positions as tools for targeted transposon tagging.
    Mol Gen Genet. 1994 Jun 15;243(6):666-73 PMID: 8028583
  11. Molecular interactions between the components of the En-I transposable element system of Zea mays.
    EMBO J. 1985 Mar;4(3):579-83 PMID: 15926216
  12. Light-induced expression of the chimeric chalcone synthase-NPTII gene in tobacco cells.
    EMBO J. 1986 Jan;5(1):1-8 PMID: 16453661
  13. Transposition in plants: a molecular model.
    EMBO J. 1985 Mar;4(3):585-90 PMID: 15926218
  14. Mobility of the maize suppressor-mutator element in transgenic tobacco cells.
    Proc Natl Acad Sci U S A. 1989 Apr;86(7):2219-23 PMID: 2538837
  15. The Cauliflower Mosaic Virus 35S Promoter: Combinatorial Regulation of Transcription in Plants.
    Science. 1990 Nov 16;250(4983):959-66 PMID: 17746920
  16. The En/Spm transposable element of Zea mays contains splice sites at the termini generating a novel intron from a dSpm element in the A2 gene.
    EMBO J. 1990 Oct;9(10):3051-7 PMID: 2170105
  17. The use of transgenic plants to understand transposition mechanisms and to develop transposon tagging strategies.
    Plant Mol Biol. 1991 Mar;16(3):449-61 PMID: 1654159
  18. Definition and characterization of an artificial En/Spm-based transposon tagging system in transgenic tobacco.
    Plant Mol Biol. 1993 Oct;23(1):157-78 PMID: 8219047
  19. About maize transposable elements and development.
    Cell. 1989 Jan 27;56(2):181-91 PMID: 2536297
  20. Pattern of somatic transposition in a high copy Ac tomato line.
    Plant J. 1992 Mar;2(2):173-9 PMID: 1338773
  21. The En mutable system in maize : III. Transposition associated with mutational events.
    Theor Appl Genet. 1970 Jan;40(8):367-77 PMID: 24435949
  22. Maize Spm transposable element has an enhancer-insensitive promoter.
    Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6355-9 PMID: 8392198
  23. High level expression of the Activator transposase gene inhibits the excision of Dissociation in tobacco cotyledons.
    Cell. 1993 Nov 5;75(3):507-17 PMID: 8221890
  24. Isolation of a gene encoding a novel chloroplast protein by T-DNA tagging in Arabidopsis thaliana.
    EMBO J. 1990 May;9(5):1337-46 PMID: 2158442
  25. Tagging and Cloning of a Petunia Flower Color Gene with the Maize Transposable Element Activator.
    Plant Cell. 1993 Apr;5(4):371-378 PMID: 12271068
  26. The maize En-1/Spm element transposes in potato.
    Mol Gen Genet. 1989 May;217(1):172-7 PMID: 2475754
  27. Mobility of the maize transposable element En/Spm in Arabidopsis thaliana.
    Plant J. 1993 Jun;3(6):773-84 PMID: 8401610
  28. Heterologous transposon tagging of the DRL1 locus in Arabidopsis.
    Plant Cell. 1993 Jun;5(6):631-8 PMID: 8392411
  29. Molecular identification and isolation of the Waxy locus in maize.
    Cell. 1983 Nov;35(1):225-33 PMID: 6313224
  30. Ac transposition from a T-DNA can generate linked and unlinked clusters of insertions in the tomato genome.
    Genetics. 1991 Nov;129(3):833-44 PMID: 1684332
  31. Genetic and molecular analysis of the Spm-dependent a-m2 alleles of the maize a locus.
    Genetics. 1987 Sep;117(1):117-37 PMID: 2822531
  32. Transpositional behavior of the maize En/Spm element in transgenic tobacco.
    EMBO J. 1989 May;8(5):1315-21 PMID: 16453881
  33. Excision of the En/Spm transposable element of Zea mays requires two element-encoded proteins.
    EMBO J. 1990 Dec;9(12):4037-44 PMID: 2174354
  34. Transposon tagging of a male sterility gene in Arabidopsis.
    Nature. 1993 Jun 24;363(6431):715-7 PMID: 8390620
  35. Expression in plants of two bacterial antibiotic resistance genes after protoplast transformation with a new plant expression vector.
    Nucleic Acids Res. 1986 Jul 25;14(14):5857-68 PMID: 3016666
  36. Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection.
    Proc Natl Acad Sci U S A. 1988 Aug;85(15):5536-40 PMID: 16593964
  37. Conserved function in Nicotiana tabacum of a single Drosophila hsp70 promoter heat shock element when fused to a minimal T-DNA promoter.
    Mol Gen Genet. 1989 Oct;219(1-2):9-16 PMID: 2559318
  38. Molecular analysis of instability in flower pigmentation of Antirrhinum majus, following isolation of the pallida locus by transposon tagging.
    EMBO J. 1985 Jul;4(7):1625-30 PMID: 16453618
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00290346
Published
1995-06-10
Pages
555-64
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00290346
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