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PMID: 1668614 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The tnpA and tnpD gene products of the Spm element are required for transposition in tobacco.

The Plant cell ·Vol. 3 ·No. 1 ·1991-01-00 ·Pages 73-85

Masson P, Strem M, Fedoroff N

Abstract

The maize Suppressor-mutator (Spm) element encodes four alternatively spliced transcripts designated tnpA, tnpB, tnpC, and tnpD. tnpA and tnpB are monocistronic, whereas tnpC and tnpD are dicistronic, and the protein-coding sequences of each transcript overlap extensively with those of one or more of the other transcripts. We have analyzed the role of the Spm-encoded gene products in element transposition by using cDNAs with a single open reading frame to (1) complement Spm elements with frameshift mutations and (2) complement each other in a tobacco transposition assay. We report that whereas the tnpA and tnpD gene products are essential for transposition, the tnpB and tnpC gene products are not. We have analyzed the structure of empty donor sites, new insertion sites, and potential transposition intermediates. We discuss the implications of our findings for the mechanism of Spm transposition.

MeSH Terms
Base Sequence DNA Transposable Elements DNA-Binding Proteins Genetic Complementation Test Models, Genetic Molecular Sequence Data Open Reading Frames Plant Proteins/genetics Plants, Toxic Polymerase Chain Reaction RNA, Messenger/genetics Repressor Proteins Sequence Homology, Nucleic Acid Tobacco/genetics
Chemicals
DNA Transposable Elements DNA-Binding Proteins Plant Proteins RNA, Messenger Repressor Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Masson P
Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210.
Strem M
Fedoroff N
References (15)
15 references, click to expand
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.3.1.73
Published
1991-01-00
Pages
73-85
Language
English
Region
England
NLM ID
9208688
PMCID
PMC159980
Subset
IM
Grants
NIGMS NIH HHS · 5-R37-GM34296 · United States
External Links
PubMed source DOI: 10.1105/tpc.3.1.73 PMC Full Text View journal details
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