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PMID: 7601858 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Evidence that operons tcb, tfd, and clc encode maleylacetate reductase, the fourth enzyme of the modified ortho pathway.

Journal of bacteriology ·Vol. 177 ·No. 13 ·1995-07-00 ·Pages 3885-9

Kasberg T, Daubaras DL, Chakrabarty AM, Kinzelt D, Reineke W

Abstract

The maleylacetate reductase from Pseudomonas sp. strain B13 functioning in the modified ortho pathway was purified and digested with trypsin. The polypeptides separated by high-performance liquid chromatography were sequenced. Alignments with the polypeptides predicted from the tfdF and tcbF genes located on plasmids pJP4 of the 2,4-dichlorophenoxyacetate-degrading Alcaligenes eutrophus JMP134 and pP51 of the 1,2,4-trichlorobenzene-degrading Pseudomonas sp. strain P51 as well as polypeptides predicted from the tftE gene located on the chromosome of the 2,4,5-trichlorophenoxyacetate-degrading Burkholderia cepacia AC1100 were obtained. In addition, the deduced protein sequence encoded by the nucleotide sequence downstream of clcD on plasmid pAC27 of the 3-chlorobenzoate-degrading Pseudomonas putida AC866 was tested for homology. Significant sequence similarities with the polypeptides encoded by the tfdF, tcbF, and tftE genes as well as the nucleotide sequence downstream of the clcD gene gave evidence that these genes might encode maleylacetate reductases. A NAD-binding motif in a beta alpha beta-element was detected.

MeSH Terms
2,4-Dichlorophenoxyacetic Acid/metabolism Amino Acid Sequence Biodegradation, Environmental Chlorobenzenes/metabolism Chlorobenzoates/metabolism Dioxygenases Genes, Bacterial/genetics Molecular Sequence Data Operon/genetics Oxidoreductases/chemistry,genetics,metabolism Oxidoreductases Acting on CH-CH Group Donors Oxygenases/metabolism Peptide Fragments/chemistry Pseudomonas/enzymology,genetics,metabolism Sequence Analysis Sequence Homology, Amino Acid
Chemicals
Chlorobenzenes Chlorobenzoates Peptide Fragments 3-chlorobenzoic acid 1,2,4-trichlorobenzene 2,4-Dichlorophenoxyacetic Acid 4-dichlorobenzene Oxidoreductases Oxygenases Dioxygenases chlorocatechol 1,2-dioxygenase Oxidoreductases Acting on CH-CH Group Donors maleylacetate reductase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kasberg T
Bergische Universität-Gesamthochschule Wuppertal, Germany.
Daubaras D L
Chakrabarty A M
Kinzelt D
Reineke W
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.13.3885-3889.1995
Published
1995-07-00
Pages
3885-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177112
Subset
IM
Grants
NIEHS NIH HHS · ES 04050-09 · United States
External Links
PubMed source DOI: 10.1128/jb.177.13.3885-3889.1995 PMC Full Text View journal details
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