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PMID: 1482270 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Maleylacetate reductase of Pseudomonas sp. strain B13: dechlorination of chloromaleylacetates, metabolites in the degradation of chloroaromatic compounds.

Archives of microbiology ·Vol. 158 ·No. 6 ·1992-00-00 ·Pages 412-7

Kaschabek SR, Reineke W

Abstract

The maleylacetate reductase of 3-chlorobenzoate-grown cells of Pseudomonas sp. strain B13 has been purified 50-fold. The enzyme converted 2-chloromaleylacetate to 3-oxoadipate with temporary occurrence of maleylacetate; 1 mol of chloride was eliminated during the conversion of 1 mol of 2-chloro- and 2,3-dichloromaleylacetate; 2 mol of NADH were consumed per mol of 2-chloro- and 2,3-dichloromaleylacetate while only 1 mol was necessary to catalyze the conversion of maleylacetate or 2-methylmaleylacetate. The maleylacetate reductase failed to use fumarylacetate as a substrate. The role of the enzyme in the chloroaromatics degradation is discussed.

MeSH Terms
Adipates/metabolism Biodegradation, Environmental Chlorides/metabolism Chlorobenzoates/metabolism Kinetics Maleates/metabolism NAD/metabolism Oxidation-Reduction Oxidoreductases/isolation & purification,metabolism Oxidoreductases Acting on CH-CH Group Donors Pseudomonas/enzymology
Chemicals
Adipates Chlorides Chlorobenzoates Maleates 3-chlorobenzoic acid NAD 3-oxoadipic acid 2,3-dichloromaleylacetate 2-chloromaleylacetate Oxidoreductases Oxidoreductases Acting on CH-CH Group Donors maleylacetate reductase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kaschabek S R
Chemische Mikrobiologie der Bergischen Universität-Gesamthochschule Wuppertal, Federal Republic of Germany.
Reineke W
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Article Info
Journal
Archives of microbiology
Abbr.
Arch Microbiol
ISSN
0302-8933
DOI
10.1007/BF00276301
Published
1992-00-00
Pages
412-7
Language
English
Region
Germany
NLM ID
0410427
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00276301 View journal details
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