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PMID: 7588602 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Subunit composition of G(o) proteins functionally coupling galanin receptors to voltage-gated calcium channels.

The EMBO journal ·Vol. 14 ·No. 19 ·1995-10-02 ·Pages 4728-37

Kalkbrenner F, Degtiar VE, Schenker M, Brendel S, Zobel A, Heschler J, Wittig B, Schultz G

Abstract

The neuropeptide galanin is widely expressed in the central nervous system and other tissues and induces different cellular reactions, e.g. hormone release from pituitary and inhibition of insulin release from pancreatic B cells. By microinjection of antisense oligonucleotides we studied the question as to which G proteins mediate the galanin-induced inhibition of voltage-gated Ca2+ channels in the rat pancreatic B-cell line RINm5F and in the rat pituitary cell line GH3. Injection of antisense oligonucleotides directed against alpha 01, beta 2, beta 3, gamma 2 and gamma 4 G protein subunits reduced the inhibition of Ca2+ channel current which was induced by galanin, whereas no change was seen after injection of cells with antisense oligonucleotides directed against alpha i, alpha q, alpha 11, alpha 14, alpha 15, beta 1, beta 4, gamma 1, gamma 3, gamma 5, or gamma 7 G protein subunits or with sense control oligonucleotides. In view of these data and of previous results, we conclude that the galanin receptors in GH3 and in RINm5F cells couple mainly to the G(0) protein consisting of alpha 01 beta 2 gamma 2 to inhibit Ca2+ channels and use alpha 01beta 3 gamma 4 less efficiently. The latter G protein composition was previously shown to be used by muscarinic M4 receptors to inhibit Ca2+ channels.

MeSH Terms
Amino Acid Sequence Animals Barium Compounds/metabolism Base Sequence Calcium Channel Blockers/pharmacology Calcium Channels/physiology Carbachol/pharmacology Cell Line Cell Nucleus Chlorides/metabolism GTP-Binding Protein alpha Subunits, Gi-Go GTP-Binding Proteins/chemistry,metabolism Galanin/pharmacology Ion Channel Gating/physiology Islets of Langerhans/cytology,physiology Microinjections Molecular Sequence Data Muscarinic Agonists/pharmacology Oligonucleotides, Antisense Pituitary Gland/cytology,physiology Rats Receptors, Galanin Receptors, Gastrointestinal Hormone/physiology
Chemicals
Barium Compounds Calcium Channel Blockers Calcium Channels Chlorides Muscarinic Agonists Oligonucleotides, Antisense Receptors, Galanin Receptors, Gastrointestinal Hormone barium chloride Galanin Carbachol GTP-Binding Proteins GTP-Binding Protein alpha Subunits, Gi-Go
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Kalkbrenner F
Institut für Pharmakologie der Freien Universität Berlin, Germany.
Degtiar V E
Schenker M
Brendel S
Zobel A
Heschler J
Wittig B
Schultz G
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-10-02
Pages
4728-37
Language
English
Region
England
NLM ID
8208664
PMCID
PMC394570
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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