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PMID: 7568167 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A nuclear hormone receptor-associated protein that inhibits transactivation by the thyroid hormone and retinoic acid receptors.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 21 ·1995-10-10 ·Pages 9525-9

Burris TP, Nawaz Z, Tsai MJ, O'Malley BW

Abstract

Nuclear hormone receptors are transcription factors that require multiple protein-protein interactions to regulate the expression of their target genes. Using the yeast two-hybrid system, we identified a protein, thyroid hormone receptor uncoupling protein (TRUP), that specifically interacts with a region of the human thyroid hormone receptor (TR) consisting of the hinge region and the N-terminal portion of the ligand binding domain in a hormone-independent manner. Interestingly, TRUP inhibits transactivation by TR and the retinoic acid receptor but has no effect on the estrogen receptor or the retinoid X receptor in mammalian cells. We also demonstrate that TRUP exerts its action on TR and retinoic acid receptor by interfering with their abilities to interact with their DNA. TRUP represents a type of regulatory protein that modulates the transcriptional activity of a subclass of the nuclear hormone receptor superfamily by preventing interaction with their genomic response elements.

MeSH Terms
Amino Acid Sequence Animals Humans Molecular Sequence Data Protein Binding Receptors, Estrogen/metabolism Receptors, Retinoic Acid/metabolism Receptors, Thyroid Hormone/metabolism Repressor Proteins/genetics,metabolism Retinoid X Receptors Ribosomal Proteins/genetics,metabolism Transcription Factors/metabolism Transcriptional Activation
Chemicals
RPL7A protein, human Receptors, Estrogen Receptors, Retinoic Acid Receptors, Thyroid Hormone Repressor Proteins Retinoid X Receptors Ribosomal Proteins Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Burris T P
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Nawaz Z
Tsai M J
O'Malley B W
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.21.9525
Published
1995-10-10
Pages
9525-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC40834
Subset
IM
Databases
GENBANK
M36072
External Links
PubMed source DOI: 10.1073/pnas.92.21.9525 PMC Full Text View journal details
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