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PMID: 7565780 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Genetic enhancement of RNA-processing defects by a dominant mutation in B52, the Drosophila gene for an SR protein splicing factor.

Molecular and cellular biology ·Vol. 15 ·No. 11 ·1995-11-00 ·Pages 6273-82

Peng X, Mount SM

Abstract

SR proteins are essential for pre-mRNA splicing in vitro, act early in the splicing pathway, and can influence alternative splice site choice. Here we describe the isolation of both dominant and loss-of-function alleles of B52, the gene for a Drosophila SR protein. The allele B52ED was identified as a dominant second-site enhancer of white-apricot (wa), a retrotransposon insertion in the second intron of the eye pigmentation gene white with a complex RNA-processing defect. B52ED also exaggerates the mutant phenotype of a distinct white allele carrying a 5' splice site mutation (wDR18), and alters the pattern of sex-specific splicing at doublesex under sensitized conditions, so that the male-specific splice is favored. In addition to being a dominant enhancer of these RNA-processing defects, B52ED is a recessive lethal allele that fails to complement other lethal alleles of B52. Comparison of B52ED with the B52+ allele from which it was derived revealed a single change in a conserved amino acid in the beta 4 strand of the first RNA-binding domain of B52, which suggests that altered RNA binding is responsible for the dominant phenotype. Reversion of the B52ED dominant allele with X rays led to the isolation of a B52 null allele. Together, these results indicate a critical role for the SR protein B52 in pre-mRNA splicing in vivo.

MeSH Terms
Alleles Amino Acid Sequence Animals Base Sequence DNA Primers/chemistry Drosophila Proteins Drosophila melanogaster/embryology,genetics Frameshift Mutation Genes, Dominant Genes, Insect Molecular Sequence Data Nuclear Proteins Phosphoproteins Point Mutation Protein Structure, Tertiary Proteins/physiology RNA Splicing RNA Splicing Factors RNA-Binding Proteins/physiology Sequence Deletion Sex Determination Analysis
Chemicals
DNA Primers Drosophila Proteins Nuclear Proteins Phosphoproteins Proteins RNA Splicing Factors RNA-Binding Proteins B52 protein, Drosophila
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Peng X
Department of Biological Sciences, Columbia University, New York, New York 10027, USA.
Mount S M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
Published
1995-11-00
Pages
6273-82
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230879
Subset
IM
Grants
NIGMS NIH HHS · GM37991 · United States
Databases
GENBANK
X58720
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