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PMID: 7565699 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Structure-function relationships of the yeast cyclin-dependent kinase Pho85.

Molecular and cellular biology ·Vol. 15 ·No. 10 ·1995-10-00 ·Pages 5482-91

Santos RC, Waters NC, Creasy CL, Bergman LW

Abstract

The PHO85 gene of Saccharomyces cerevisiae encodes a cyclin-dependent kinase involved in both transcriptional regulation and cell cycle progression. Although a great deal is known concerning the structure, function, and regulation of the highly homologous Cdc28 protein kinase, little is known concerning these relationships in regard to Pho85. In this study, we constructed a series of Pho85-Cdc28 chimeras to map the region(s) of the Pho85 molecule that is critical for function of Pho85 in repression of acid phosphatase (PHO5) expression. Using a combination of site-directed and ethyl methanesulfonate-induced mutagenesis, we have identified numerous residues critical for either activation of the Pho85 kinase, interaction of Pho85 with the cyclin-like molecule Pho80, or substrate recognition. Finally, analysis of mutations analogous to those previously identified in either Cdc28 or cdc2 of Schizosaccharomyces pombe suggested that the inhibition of Pho85-Pho80 activity in mechanistically different from that seen in the other cyclin-dependent kinases.

Related Genes
PHO85
MeSH Terms
Acid Phosphatase/biosynthesis Amino Acid Sequence CDC28 Protein Kinase, S cerevisiae/genetics,metabolism Conserved Sequence Cyclin-Dependent Kinases/chemistry,genetics,metabolism Cyclins/metabolism Enzyme Repression Fungal Proteins/metabolism Genetic Complementation Test Molecular Sequence Data Mutagenesis Recombinant Fusion Proteins/biosynthesis Repressor Proteins Saccharomyces cerevisiae/enzymology,genetics,growth & development Saccharomyces cerevisiae Proteins Sequence Alignment Structure-Activity Relationship
Chemicals
Cyclins Fungal Proteins PHO80 protein, S cerevisiae Recombinant Fusion Proteins Repressor Proteins Saccharomyces cerevisiae Proteins CDC28 Protein Kinase, S cerevisiae Cyclin-Dependent Kinases PHO85 protein, S cerevisiae Acid Phosphatase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Santos R C
Department of Microbiology, Medical College of Pennsylvania, Philadelphia, USA.
Waters N C
Creasy C L
Bergman L W
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36 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.10.5482
Published
1995-10-00
Pages
5482-91
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230798
Subset
IM
Grants
NIGMS NIH HHS · GM41620 · United States
External Links
PubMed source DOI: 10.1128/MCB.15.10.5482 PMC Full Text View journal details
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