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PMID: 7565605 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

DNA supercoiling in a thermotolerant mutant of Escherichia coli.

Molecular & general genetics : MGG ·Vol. 248 ·No. 4 ·1995-08-30 ·Pages 417-22

Friedman SM, Malik M, Drlica K

Abstract

A spontaneously occurring, nalidixic acid-resistant (NalR), thermotolerant (T/r) mutant of Escherichia coli was isolated. Bacteriophage P1-mediated transduction showed that NalR mapped at or near gyr A, one of the two genes encoding DNA gyrase. Expression of gyrA+ from a plasmid rendered the mutant sensitive to nalidixic acid and to high temperature, the result expected for alleles mapping in gyrA. Plasmid linking number measurements, made with DNA from cells grown at 37 degrees C or shifted to 48 degrees C, revealed that supercoiling was about 12% less negative in the T/r mutant than in the parental strain. Each strain preferentially expressed two different proteins at 48 degrees C. The genetic and supercoiling data indicate that thermotolerance can arise from an alteration in DNA gyrase that lowers supercoiling. This eubacterial study, when coupled with those of archaebacteria, suggests that DNA relaxation is a general aspect of thermotolerance.

Related Genes
gyrA
MeSH Terms
Chromosome Mapping DNA Topoisomerases, Type II/genetics,metabolism DNA, Bacterial DNA, Superhelical Escherichia coli/genetics,growth & development,isolation & purification Mutation Plasmids Temperature
Chemicals
DNA, Bacterial DNA, Superhelical DNA Topoisomerases, Type II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Friedman S M
Department of Biological Sciences, Hunter College of The City University of New York, NY 10021, USA.
Malik M
Drlica K
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32 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF02191641
Published
1995-08-30
Pages
417-22
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIAID NIH HHS · AI 35257 · United States
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1007/BF02191641
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