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PMID: 7561694 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Successful T cell priming in B cell-deficient mice.

The Journal of experimental medicine ·Vol. 182 ·No. 4 ·1995-10-01 ·Pages 915-22

Epstein MM, Di Rosa F, Jankovic D, Sher A, Matzinger P

Abstract

B cells are an abundant population of lymphocytes that can efficiently capture, process, and present antigen for recognition by activated or memory T cells. Controversial experiments and arguments exist, however, as to whether B cells are or should be involved in the priming of virgin T cells in vivo. Using B cell-deficient mice, we have studied the role of B cells as antigen-presenting cells in a wide variety of tests, including assays of T cell proliferation and cytokine production in responses to protein antigens, T cell killing to minor and major histocompatibility antigens, skin graft rejection, and the in vitro and in vivo responses to shistosome eggs. We found that B cells are not critical for either CD4 or CD8 T cell priming in any of these systems. This finding lends support to the notion that the priming of T cells is reserved for specialized cells such as dendritic cells and that antigen presentation by B cells serves distinct immunological functions.

MeSH Terms
Animals Antigen Presentation Antigen-Presenting Cells/immunology B-Lymphocytes/immunology CD4-Positive T-Lymphocytes/immunology CD8-Positive T-Lymphocytes/immunology Cytokines/analysis Female Fluorescent Antibody Technique Immunoglobulin mu-Chains/genetics Lymphocyte Activation Male Mice Mice, Inbred C57BL Mice, Mutant Strains Schistosomiasis mansoni/immunology Skin Transplantation T-Lymphocytes/immunology
Chemicals
Cytokines Immunoglobulin mu-Chains
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Epstein M M
Laboratory of Cellular and Molecular Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
Di Rosa F
Jankovic D
Sher A
Matzinger P
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.182.4.915
Published
1995-10-01
Pages
915-22
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2192294
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.182.4.915 PMC Full Text View journal details
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