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PMID: 7540747 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nonselective cationic currents elicited by extracellular ATP in human B-lymphocytes.

Pflugers Archiv : European journal of physiology ·Vol. 429 ·No. 5 ·1995-03-00 ·Pages 691-8

Bretschneider F, Klapperstück M, Löhn M, Markwardt F

Abstract

Adenosine 5'-triphosphate-(ATP)-induced whole-cell currents were studied in human B-lymphocytes, transformed by the Epstein-Barr virus, by means of the tight-seal voltage-clamp technique. During bath application of ATP, the membrane conductance was increased. The change of membrane conductance occurred within milliseconds. The dose response relationship for the ATP(4-)-elicited membrane current (Ip) was fitted by the Hill function with a Hill coefficient of 1 and a KD value of 0.2 mmol/l. Adenosine, as well as the Mg(2+)-bound form of ATP, did not effect the membrane conductance. Ip did not desensitize within 1 min and could be evoked repeatedly up to 100 times in 1 cell in the presence of the G-protein blocker Guanosine 5'-o-(2-thiodiphosphate) (GDP [beta S]). Therefore, it seems that ion channels in form of P2Z-purinoceptors are involved in the observed effects. The permeability (P) sequence for cations carrying Ip was PCa:PK:PCs:PNa:PTRIS = 35:2:1.2:1:0.1. The reversal potential of IP was not changed by substitution of intracellular Cl- for aspartate, indicating that anions are not involved in the purinoceptor-dependent conductance. A single-channel conductance of P2Z-receptor-dependent ion channels of about 3 pS was determined by noise analysis of Ip.

MeSH Terms
Adenosine Triphosphate/pharmacology B-Lymphocytes/drug effects,metabolism Cell Membrane Permeability/drug effects Electrophysiology Extracellular Space/drug effects,metabolism Humans Ion Channels/drug effects,metabolism Kinetics Ligands Patch-Clamp Techniques
Chemicals
Ion Channels Ligands Adenosine Triphosphate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bretschneider F
Martin-Luther-University Halle-Wittenberg, Julius-Bernstein-Institute for Physiology, Halle/S., Germany.
Klapperstück M
Löhn M
Markwardt F
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00373990
Published
1995-03-00
Pages
691-8
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00373990 View journal details
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