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PMID: 7532679 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Breakdown of B cell tolerance in a mouse model of systemic lupus erythematosus.

The Journal of experimental medicine ·Vol. 181 ·No. 3 ·1995-03-01 ·Pages 1157-67

Roark JH, Kuntz CL, Nguyen KA, Caton AJ, Erikson J

Abstract

Anti-DNA antibodies, specifically those that stain nuclei in a homogenous nuclear (HN) fashion, are diagnostic of systemic lupus erythematosus (SLE) and the MRL-lpr/lpr SLE murine model. We have used a heavy chain transgene that increases the frequency of anti-HN antibodies to address whether their production in SLE is the consequence of a defect in B cell tolerance. Anti-HN B cells were undetectable in nonautoimmune-prone transgenic mice, but in MRL-lpr/lpr transgenic mice their Ig was evident in the sera and they were readily retrievable as hybridomas. We conclude that nonautoimmune animals actively delete anti-HN-specific B cells, and that MRL-lpr/lpr mice are defective in this process possibly because of the lpr defect in the fas gene.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Antinuclear/biosynthesis Antigens, Surface/genetics,physiology B-Lymphocytes/immunology Base Sequence DNA, Single-Stranded/immunology Genes, Immunoglobulin Hybridomas/immunology Immune Tolerance Immunoglobulin Variable Region/chemistry Lupus Erythematosus, Systemic/genetics,immunology Mice Mice, Inbred BALB C Mice, Transgenic Molecular Sequence Data fas Receptor
Chemicals
Antibodies, Antinuclear Antigens, Surface DNA, Single-Stranded Immunoglobulin Variable Region fas Receptor
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Roark J H
Wistar Institute, Philadelphia, Pennsylvania 19104.
Kuntz C L
Nguyen K A
Caton A J
Erikson J
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53 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.181.3.1157
Published
1995-03-01
Pages
1157-67
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2191913
Subset
IM
Grants
NIAID NIH HHS · 5R01 AI24541-08 · United States
NIAID NIH HHS · 5R01 AI32137-04 · United States
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GENBANK
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External Links
PubMed source DOI: 10.1084/jem.181.3.1157 PMC Full Text View journal details
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