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PMID: 1518867 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Induction of tolerance to an IgG autoantibody.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 17 ·1992-09-01 ·Pages 8332-6

Offen D, Spatz L, Escowitz H, Factor S, Diamond B

Abstract

Nonautoimmune mice transgenic for the heavy chain of an IgG2b anti-double-stranded-DNA antibody express the transgene in lymphoid organs and display partial allelic exclusion of this gamma 2b transgene. The spleens of these mice are characterized by marked B-cell depletion. Although there are B cells in these mice that express the transgene and recognize double-stranded DNA, they are anergic in vivo. Recovery from the state of anergy occurs in vitro after lipopolysaccharide stimulation. Thus this transgenic model demonstrates the induction of self tolerance to an IgG autoantibody.

MeSH Terms
Animals Antibodies, Antinuclear/genetics,immunology B-Lymphocytes/immunology Gene Expression Genes Immune Tolerance Immunoglobulin G/genetics,immunology Kidney/pathology Lipopolysaccharides/immunology Mice Mice, Transgenic RNA, Messenger/genetics Spleen/pathology
Chemicals
Antibodies, Antinuclear Immunoglobulin G Lipopolysaccharides RNA, Messenger
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Offen D
Department of Microbiology, Albert Einstein College of Medicine, Bronx, NY 10461.
Spatz L
Escowitz H
Factor S
Diamond B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.17.8332
Published
1992-09-01
Pages
8332-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49912
Subset
IM
Grants
NIAID NIH HHS · AI-10702 · United States
NIAMS NIH HHS · AR-32371 · United States
NCI NIH HHS · CA-13330 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.17.8332 PMC Full Text View journal details
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