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PMID: 3480535 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structure and function of anti-DNA autoantibodies derived from a single autoimmune mouse.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 24 ·1987-12-00 ·Pages 9150-4

Shlomchik MJ, Aucoin AH, Pisetsky DS, Weigert MG

Abstract

Four monoclonal anti-DNA antibodies derived from a single autoimmune MRL/lpr mouse were studied. Three of these antibodies showed similarities in DNA binding; the fourth had a much higher specific activity for single-stranded DNA and, in addition, was unique in binding double-stranded DNA and cardiolipin. Complete nucleotide sequences of heavy- and light-chain variable regions demonstrated that all four antibodies are clonally related. The sequences also showed numerous somatic mutations, the distribution of which suggests that positive selection by antigen operated on these clonally related autoantibodies.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Monoclonal/immunology Antibody Specificity Autoantibodies/immunology Base Sequence Cardiolipins/immunology DNA/immunology Genes, Immunoglobulin Immunoglobulin Variable Region/genetics Lupus Erythematosus, Systemic/immunology Mice Mice, Mutant Strains Molecular Sequence Data Mutation
Chemicals
Antibodies, Monoclonal Autoantibodies Cardiolipins Immunoglobulin Variable Region DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shlomchik M J
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, PA 19111.
Aucoin A H
Pisetsky D S
Weigert M G
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.24.9150
Published
1987-12-00
Pages
9150-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299710
Subset
IM
Databases
GENBANK
J03595, M18233, M18234, M18235, M18236, M18237, M18238, M18239, M22450, M22451
External Links
PubMed source DOI: 10.1073/pnas.84.24.9150 PMC Full Text View journal details
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